Lab Exercise At Room Temperature Equal

Purpose: To learn about the international system of units (SI), to become familiar with common lab equipment and techniques, to gain proficiency in determining volume, mass, length, and temperature of a variety of items using common laboratory measurement devices, to learn to combine units to determine density and concentration, and to use laboratory equipment to create serial dilutions and determine the density and concentration of each dilution.

Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a volumetric flask, graduated pipet, pipet bulb, scale, and glass beaker to determine concentrations and densities of various dilutions.

Perform the following with three 0.05g samples of unknown and the following solvents: methanol, water and toluene

Experiment 2 “Density” was about how to measure the mass and volume and determine the density of water, alcohol, and a solid. For this lab, we begin by calculating the mass of empty graduated cylinder and the mass of 25 mL of tap water. After taking the mass of water and cylinder, we record it on “Density of Water: Data Table”. Since we did not have enough time of complete the whole lab, so we skipped the procedure to measure the density of alcohol. We jumped to the next procedure, which was density of a solid. For that we used a copper and it density 8.95. For this lab, my partner and I did not make any mistakes or errors. But, for better improvement I think we should have more time to complete the whole lab. I felt like we was rushing through

An automatic pipet was used to measure 0.450 mL water and 0.165 mL acetic anhydride and was added to the conical vial. A spin vane was placed into the vial and an air condenser was attached.

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

2. In order to calculate the density of a solid or liquid sample, what measurements are needed?

Abstract: This experiment introduced the student to lab techniques and measurements. It started with measuring length. An example of this would be the length of a nickel, which is 2cm. The next part of the experiment was measuring temperature. I found that water boils around 95ºC at 6600ft. Ice also has a significant effect on the temperature of water from the tap. Ice dropped the temperature about 15ºC. Volumetric measurements were the basis of the 3rd part of the experiment. It was displayed during this experiment that a pipet holds about 4mL and that there are approximately 27 drops/mL from a short stem pipet. Part 4 introduced the student to measuring

The volume of a small test tube and a thin-stemmed pipet were determined in this section of the lab. Water was poured into a small test tube until the water reached the very top edge of the test tube. The test tube was then emptied into a plastic 25 mL graduated cylinder and volume was measured and recorded into data table 3. A think-stemmed pipet was completely filled with water. Drops were carefully counted and emptied into the empty plastic 25 mL graduated cylinder until the water level reached 1 mL. The number of drops in 1 mL was recorded into data table 3. The thin-stemmed pipet had a total volume of 4 mL and that was also recorded into data table 3.

The purpose of this experiment is to familiarize oneself with the general procedures determining a partition coefficient at the microscale level and learn in weighing milligram quantities of materials on an electronic balance, the use of automatic pipets, the use of transfer pipet, and the use of a vortex mixer. Also, to familiarize oneself with extraction

The purpose of this experiment is to identify an unknown substance by measuring the density and boiling point. I will be able to conclude which substance is my own from a list of known options stating what its real boiling point and density is.

Group 1 received 107% for 0.0m, 109% for 0.25m, 112% for 0.5m, and 126% for 0.75m. Group 2 received 100% for 0.0m, 107% for 0.25m, 116% for 0.5m, and 117% for 0.75m. Group 3 received 100% for 0.0m, 106% for 0.25m, 114% for 0.5 m, and 117% for 0.75m. Group 4 received 100% for 0.0m, 106% for 0.25m, 113% for 0.5m, and 116% for 0.75m. Group 5 received 98% for 0.0m, 105% for 0.25m, 110% for 0.5m, and 115% for 0.75m. Group 6 received 104% for 0.0m, 108% for 0.25m, 111% for 0.5m, and 104% for 0.75m. The trend line that occurred for the rate of osmosis was an upward increase as the solute concentration increased.

The micropipette is used to collect very small amounts of liquids that an average pipette would not be able to accurately collect. For this lab, one needs to gather two tubes which will each contain 250µL of solution. One tube will then be filled with the +pGLO plasmid and the other will contain -pGLO. The pGLO plasmid is NOT transmitted into the -pGLO tube. The solution the pGLO plasmid is in is called transformation solution and is used to soften the membrane of the E. coli cells with the calcium chloride it contains. The tubes must remain in an ice bath for ten minutes to bring their temperature down so that they will react strongly when exposed to the heat shock. Whenever researchers are transferring the E. coli bacteria from tube to tube, they must wear protective gloves and sterile loops provided to them. Researchers need to obtain and label four plates: +pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, and -pGLO LB. Amp stands for ampicillin and ara stands for arabinose. Their plates contain a base gelatin of Luria-Bertani broth. Then, once the tubes are very cold on ice, researchers place the tubes in 42-degree celsius water for fifty seconds exactly in order to activate the heat shock of the E. coli cells, prompting their membranes to become more permeable and susceptible to accepting the pGLO plasmid. After the

After that, it is crucial to convert the mass to volume. The water density at a temperature will aid this process. The compliance of the Volume Occupied by 1.000g of Water Weigh in Air table is deemed necessary throughout the comparison.

Methods: There are ten experiments are perform in lab number from 2 to 11. (number 1 is omitted). Number 2: Using the dropper, place 2-3 drops of water on the glass slide labeled “water”. Lay the second glass slide over the first glass slide. Try to pull them apart without sliding the glass. Record the observations. Repeat