Lab Experiment: The Effectiveness of Different Antibiotics on Bacteria

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1. To investigate the effect of different antibiotics on bacteria

2. To develop problem solving and experimental skills, for example, information is accurately processed and presented, experimental procedures are planned, designed and evaluated properly, producing valid results, recording results, and valid conclusion is drawn.
3. To develop the aseptic techniques for preparing agar plates and bacterial culture.
Problem statement
Do antibiotics have the same effect on killing different types of bacteria?

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment
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Preventing the formation of cross-linking in the cell wall
3. Damaging the cell membranes of bacteria
4. Preventing the transcription or translation of microbial genes (mechanism for tetracyclines)
5. Stopping the bacterial DNA from coiling up

Examples of antibiotics that are commonly used in infection treatment include: gentamycin, tetracycline, streptomycin, and carbenicillin


Bacillus subtilis is rod-shaped and has the ability to form a tough, protective endospore, allow the organism to tolerate extreme conditions. Some uses of Bacillus subtilis includes using as a model organism for laboratory studies. It can also be genetically modified to convert explosives into harmless compound of nitrogen, water and carbon dioxide. (Wikipedia, 2010)

A close up view of B. subtilis bacterium. Source:

Escherichia coli are commonly found in lower intestine of warm blooded animals. Most E. coli are harmless. The bacteria can be grown easily and its genetics are relatively simple and easy to study, making it the best-studied prokaryotic organism. Certain strains of E. coli can produce toxins that can cause food poisoning when eating unwashed vegetables. (Wikipedia, 2010)

Escherichia coli. Source:

Aseptic techniques
Aseptic techniques refer to the procedures that performed under

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