Lab Investigation : Cell Purple Dye Solution

From this graph and chart we can see that the higher the concentration the higher the absorbance, all the different concentrations were tested at the same wavelength (625nm). Also we can determine our unknown substances concentration by using the absorbance we got for it. The red dot on the graph followed by the line towards the horizontal axis indicates that the concentration of fast green was 34% or 5.1x10-3.

3ml of sample was taken first flask at 4 minutes and added to the appropriate tube of sodium hydroxide, from the second flask at 4.5 minute and so on, each flask was sampled at 30 second intervals. The sampling was then repeated starting at 8,12,16 minutes. The final sample from the last flask was taken at 18.5 minutes. Once the sampling was completed, measurements of absorbance were obtained for solution in each tube at 405 nm.

The procedures for experiment A, B, and C all start the same. The first step is to put on goggles and get the data collection device set properly. The labquest needs to be plugged into the colorimeter accurately so that a click is heard when putting it in. The labquest needs to be reading digitally and the colorimeter needs to be set to 635 nm. Then shake the chloroplast solution and take a clean cuvette and fill it with 3 mL of distilled water, 3 drops of the chloroplast solution, and cap it. This is used as a blank to calibrate your labquest. Double check that the labquest is reading absorbance, this assures that the colorimeter is plugged into the labquest accurately. Insert the blank into the colorimeter and hit the calibration button. Take out the blank and empty it. The labquest is now set to experiment with. Make sure that the heat bank is set in front of the lamp and that the lamp is on. The cuvette must be placed on the opposite side of the heat bank in the path of light in the box so that no other light can interfere with the experiment.

The same solution of 0.5 ml BSA was then added from test tube 1 to the test tube 2 after being properly mixed, and from test tube 2 the solution was being added to test tube 3, and so forth all the way up to test tube 5, with the same exact procedure. From the last tube, we then disposed the 0.5 ml solution. After above procedures, we now labeled another test tube “blank”; 0.5 ml blank distilled water was purred into the tube with the serial dilution of 1:10. We also had a tube C labeled “unknown” with the same 0.5 ml of solution. And after adding 5ml of Coomassie Blue to each tube (1-5) and to the blank, the result of absorbance was read at 595 nm.

For this experiment, the amounts of Red 40 and Blue 1 were quantified in six different Kool-Aid samples through the use of a spectrophotometer. This was completing by performing serial dilutions on both dyes, Red 40 and Blue 1, and then creating calibration curves for each of the six samples. The absorbance and maximum wavelength values were obtained from the spectrophotometer for each individual drink sample. Beer’s Law was used to discover the concentration of

In the experiment I performed it was proven that, the moles of a solute, the volume of the solvent, and the molarity of an aqueous solution share a mathematical relationship. Solutions, solutes and molarity are all terms that are crucial to chemistry. A solution is defined as a liquid mixture in which the solute is distributed into the major component, and a solute is defined as the minor component within a solution. The molarity of a solution is the number of moles of solute per liter of solution and it is used to express the concentration of a solution. Both the solute amount and the solution volume can be determined in experiments to aid finding the solution concentration or molarity of a substance that they are included in. I state confidently

The purpose of this experiment is to prepare Allura Red standard solutions, use a colorimeter to measure the absorbance value of each standard solution, find the relationship between absorbance and concentration of a solution and use the results of this experiment to determine the concentration of Allura Red

The Beers Law calibration experiment used many concentrations of crystal violet solutions. Each of these solutions were test and analyzed in order to determine the absorbance of each concentration The results were than graphed and produced a slope of 1.00E05 with an intercept of -2.21E-02.

Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample

Our purpose in this lab was to create four solutions of the same components and different concentration. I found the concentration in Molarity (mol/liter). I would dilute a solution to create new solutions, using the formula M1xV1=M2xV2 where M=molarity and V=volume. In this lab, there were four solutions, Solution A, Solution B, Solution C, and Solution D. The solute or substance that dissolves was sugar and the solvent or substance that the solute dissolves in was water. For Solution A, I weighed 3.42 g sugar and diluted to 100 mL to get a concentration of 0.10 M and the color of bright red. For Solution B, I weighed 0.342 g sugar and diluted to 100 mL to get a concentration of 0.010 M and the color of pink. For Solution C, I measured 10

The values of color absorbance are effective because color absorbance has a linear relationship with concentration values, which in turn, allows us to easily find concentration values for many solutions. Beer’s law describes this phenomenon since the absorbance is directly proportional to concentration. We observed that as the color absorbance increased, the concentration of the FeSCN2+ complex ion increased. This is because as the FeSCN2+ concentration increases, the blood-red color becomes darker due to more presence of the blood-red FeSCN2+ ion. Therefore, the color absorbance increases because there is more blue color absorbed by the darker red color. We then graphed the absorbance and concentration values and created a line of best fit. Using the line of best fit, we were able to predict the equilibrium concentrations of the FeSCN2+ solutions and find the change required to reach equilibrium. Since we already knew the initial concentration of FeSCN2+ and since we already found the equilibrium concentration of FeSCN2+, we can calculate the change in equilibrium. Using this data, we were able to calculate the equilibrium concentration of all of the species in this lab, since we already knew the change from the initial concentration to the equilibrium change. Q is less than K because there was no initial concentration of FeSCN2+, but after the system reached

Figure 2: Dye percents versus absorbance in a control, 10%, 20%, and 30% azide solutions.

The Uptake of Neutral red dye by saccharomyces cerevisiae in the presence of a metabolic inhibitor

concentration, record the absorbance readings at a fixed wavelength, and plot the absorbance vs. concentration data. The wavelength of 520 nm was selected for experiment Part

Dye synthesis usually has the strongest effect on the final product because the chemical structure defines the fundamental performance of the dye. Small performance modifications can usually be achieved using additional techniques, but large changes become difficult or impossible. For example, changing the dye absorption from purple to blue using an additive is often possible, but a change from purple to orange is quite unlikely. Through synthesis, a large color change (or any dramatic property change) is much more feasible.