Essay Lab Module 1

The colonies were smooth, translucent, and had a white brownish color. The Gram stain resulted in Gram positive cocci. After the Gram stain was completed, the bacteria were streaked on a Mannitol-Salt Agar plate and a Catalase test was performed. After these test were completed a Phenol Red Dextrose Fermentation tube was inoculated, and a SIM Tube inoculated.

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

Over the years, there have been several studies published on the health effect of yogurt and the bacteria that companies use to produce yogurt. There have been studies done on animals and humans to investigate the benefits. According to The American Journal of Clinical Nutrition, “the United States uses lactic acid producing bacteria known as (LAB) which

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop slide, and morphology identification. The unknown bacterium, which was contaminated with Serratia marcescens, was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria, had the appearance of circular form, convex elevation, entire margin, elongated cocci. The tests than showed that the bacteria was gram-negative, non-motile,

Purpose of Bergy’s Manual: based on ribosomal RNA sequences, which presumably reflect phylogenetic (evolutionary) relationships. Used for the identification of prokaryotes. 2nd edition on classification of prokaryotes.

(Harley, 2011, pp. 102-103) Sucrose and lactose serve as a fermentable carbohydrate sources which promote its growth of fecal forms and provide color change differentiation. (Harley, 2011, p. 104) Therefore, if E. coli is being platted on the EMB, after plate’s incubation period, it should produce a green metallic sheen on the agar due to the bacteria being a strong fermenter. My unknown bacteria tested positive for growth, and agar was fermented reddish burgundy in color. Subsequently, the unknown bacteria was later inoculated with a sterilized loop into the liquid tryptic soy broth and incubated at an appropriate temperature. Its results were used for proper identification of turbidity, spots and flocculation. (BD™ Tryptic Soy Broth (TSB), 2008) The results of the unknown were cloudiness and some settlement on the bottom of the tryptic soy liquid. The next step was conducted to find out if all the bacteria, as well as the unknown culture, required oxygen for growth, varying from an aerobic environment, where bacteria needs oxygen to grow, to facultative anaerobic environment, where bacteria will grow either with or without oxygen but better in its presence. All the bacteria, along with the unknown, were separately inoculated and tested. My unknown culture results tested positive for growth in facultative anaerobic environment. In the next sequence of lab experiments, the results of unknown bacteria were determined by glucose fermentation,

The purpose of this experiment is to distinguish and indentify an unknown bacterium. There are several tests that can help one eliminate and narrow down the options. The most useful test, and the very first one done, is a gram stain. This test will tell whether the bacterium is gram-positive or gram-negative. After the type of gram stain is identified, the tester has a wide array of differentiating tests at their disposal. Based on the results from these tests, and the numerous others that are available, one can accurately establish the identity of an unknown bacterium.

For this experiment, I utilized unknown number three which I later identified as Staphylococcus epidermis. I concluded that the unknown organism was Staphylococcus epidermis based on numerous tests performed in the laboratory which I will discuss in detail throughout this paper. One of the first tests performed was the Gram Stain. The Gram Stain

Ocular Lens - The ocular lens is the lens you look in closet too your eyes. It helps magnify the specimen as well.

Microbiology report Aim: To investigate how the pH of yogurt is being affected by keeping it in different environments and at different temperatures. Method: 1. Prepare five specimen screw in jars. 2. Take a ruler and mark 3 cm on the jar.

These are gram positive bacteria which are found in acidic condition of pH 4 to 4.5 – acidophilic.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.

B. Label the diagram of the pocket microscope in Figure 4. Use the following terms: eyepiece,