Lab Report Digestion

The filter paper was then observed to see if it changed blue or not, in order to see if the bacteria produced cytochrome c oxidase. The final test used in the experiment was an API test. To begin the API test, a solution with bacteria and 5 mL of sterile saline, had to be made with a turbidity the same as the McFarland No. 3 (BaSO4) standard. This was done by adding loopfuls of bacteria to the saline solution, mixing the solution on the vortex, and then comparing the turbidity to the McFarland No. 3 standard, until the tubes were both at the same cloudiness. This created solution was then used in the API test by adding specified amounts to each of the microtubes on the API strip. For each of the microtubes whose names were not underlined or boxed, the tubes were filled to where the microtubes met the capsule. In the microtubes whose names were underlined, the microtubes were slightly underfilled, and then the capsule was filled with mineral oil in order to create and anaerobic environment. The last of the microbes were the ones whose names were boxed. In each of these the microtube and the capsule were filled all the way up with the bacteria. The API test strip was then placed in the 37°C incubator for 20 hours. After this time, observations were made about each of the different microtubes based on a given summary of results chart for the API test. A select number of microtubes had

Next, add enough bromothymol blue solution to each test tube until the water is blue. Next, using a straw, blow into the four test tubes until the color blue turns to the color of a yellowish/green. Then cut a sprig of Elodea from the plant and place one sprig into each of the four test tubes. Then the test tubes were sealed with a stopper. Two of the test tubes will be labeled light and placed into sunlight for about 2 hours. Then the other two test tubes will be placed into complete darkness for about 2 hours. After time is up, record the color of the solution from the four test tubes (Gunstream,

The test tube was labeled with the bacteria identifying number. The cap on test tube was removed, and the lip of the test tube was flamed. Next, the Bunsen burner was used to sterilize the inoculating loop. Then, bacteria were picked up from the working plate with the loop and the agar was inoculated. The loop was then re-flamed. Finally, the plate was placed in the 37˚C incubator and left to sit for 48 hours and any changes in color were observed. A negative result appears green, and a positive result appears blue. This is because it tests for the organism’s ability to use citrate as its sole source of carbon, and if it does then it produces ammonia and ammonium hydroxide which make the medium basic, changing the green agar blue (Leboffe & Pierce,

3. What do you think would happen if you reduced the incubation time to 30 minutes for tube 5? _This would reduce digestion in tube 5.__

The experiment was divided over the span of six days. For this procedure, Cav1.2 will be mainly referred to. On a Thursday, start with clean test tubes, the Luria broth, and either the Cav1.2, Connexin 43, or N-Cadherin (if a

We then recorded the initial color. We placed each tube in boiling water for one minute and recorded the color results and gave our conclusion. To test for starch using Lugol’s solution, we reused the test tubes and added a squirt of the solution. We recorded the final color and then our conclusion for each content. To test for lipids using paper towels, we placed a drop of solution and we let it stand for one minute. We then recorded our observation, if it was dry or not dry and wrote our conclusion for each sample. To test for proteins using Biuret’s reagent, we added a squirt of stock solution plus a few drops of Biuret’s solution. We wrote the initial color. We then shook the solution and waited for two minutes before recording the results. After the two minutes, we wrote the final color and conclusion for each content. For the unknowns, we wrote the odor and appearance of each content and then tested the benedict’s, starch, lipid, and protein test and wrote our conclusion.

4. After 3 minutes, test for the presence of sugar formation in both of the wells. Wait 10 seconds, then observe and record any color change in Table 4. The indicator changes color in varying degrees, from the initial pink to dark purple in the presence of increasing concentrations of glucose. A color change after 10 seconds is indicative of glucose production from the digestion of starch.

A positive test is given by growth surrounded by a deep blue color, whereas a negative test shows little growth without any change in the color. The tube must be placed in an incubator at 37ºC for 48

Pour the yeast solution so that there is an equal amount in each of the four test tubes. 5. Add 1⁄2 packet of sugar to test tube 1 and the other half to test tube 2. These tubes will be your experimental group. Do not add sugar to tubes 3 and 4.

Test tubes two and three were labeled 2 and 3. Labeling the test tubes are vital so that you know you are putting the right solution in the right tube. First three pipettes was opened. The first pipette was filled with 0.1 mL of guiacol, the second pipette was filled 0.2 mL hydrogen peroxide and the third pipette was filled with 4.7 of distilled water. The first solution was dropped into tube 1.

Starch (representing a plant) was put into a small beaker; the grayish white color was recorded and the test strips revealed the glucose level to be normal. Amylase was added to the small beaker as well, and the mixture was stirred. The color of the solution became clear and the glucose level was recorded to be 250 mg/dL. The end of one of the dialysis tubes was sealed with the dialysis

The digestive system is the process that involves the mouth, esophagus, stomach, small intestine, large intestine and rectum. For the body to function, the body`s cells needs energy and that energy will come from the food we eat. All the food we eat contains nutrients, carbohydrates, glucose, lipids, protein and many more other substances. The only way the body can absorb and convert these nutrients into energy to our cells is through breaking down the large molecules into smaller pieces and moist by the action of enzymes.

Experiment 7 “Carbohydrates” and the purpose for this lab is to become familiar with the properties of different classes of carbohydrates. Test such properties of carbohydrates as solubility, reduction, and hydrolysis. To do part A (solubility), begin by adding 10 mls of distilled water to three test tubes and add 2 grams of starch to one tube, 2 grams of glucose to the second, and 2 grams of sucrose to the third test tube. Shake it well and record it on the data sheet. Part B (benedict’s test), place 5 clean test tube in a test tube rack and add 3 ml of benedict’s solution to each and label 1-5. Add 2 mls of a 5% solution of: glucose (1), lactose (2), maltose (3), fructose (4), and sucrose (5), and place all of them into boiling water for

When the enzyme activity is maximized, starch will break down into more monosaccharide than other solution, and when it meets Benedict’s Reagent, monosaccharide will react with Benedict’s reagent and change color into orange. Since most of Benedict’s reagent reacted, the supernatant in the centrifuge tube after centrifuged will have less blue color and more clear. If the solution is measured in the spectro-vis spectrometer with 635nm, which is orange color, less light will be absorbed into the solution and the record will show the lowest

4.14. The accuracy of the data is dependent on the correct controls being in place, Ensuring temperate stays the same throughout all solutions, making sure that the solutions are correct that the weight and size of the eggs are the measured correctly and that when the eggs are removed they are removed at the same time. Some of the eggs were not completely submerged in the solution this may cause some errors in the experiment and may have caused varying results. The eggs were of different sizes this also will cause the results to vary, Gathering eggs of exact size and weight would have ensured the results were correct. The eggs may not have been dried enough also causing results to vary; this can be fixed by ensuring that the eggs are thoroughly dried. The eggs were taken out at a time around about 24 hours, having an exact time to remove the eggs would have eliminated errors.