Essay about Lab Report

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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4, 0.8, 1.2, 1.6, 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 microliters of bradford assay reagent, which indicates the amount of protein present…show more content…
Gel electrophoresis method. Qualitative analysis shows protein concentrations in kidney, heart, and liver. 1-6 are kidney tissue. 7-14 are liver and 15-19 are the heart tissue. The protein/DNA ratio indicated a greater ratio in the heart homogenate in comparison to the liver and kidney. The relative cell size in the heart homogenate could be inferred to be much greater because of such a high ratio. (Table 1) Homogenates Protein concentration (mg/ml) DNA concentration (μg/ml) Protein/DNA Ratio (mg/μg) Average standard deviation (Protein concentration) Heart Liver Kidney 5.915 3.224 2.709 0.55 3.39 6.54 3.28 0.78 0.44 2.490366439 0.310940777 0.737809895 Table 1 Concentration of protein, DNA, and ratio of the two in heart, kidney and liver of bos taurus. Average standard deviation of protein concentration is also shown. Triglyceride concentrations in the liver are much higher than the heart and kidney. The differences between triglyceride and glycerol concentrations were triglyceride includes the free glycerol plus the glycerol. Pure glycerol concentrations were not determined because of reagent availability. The triglyceride concentrations were assumed to be proportional to glycerol concentrations. (Figure 2) -1143002095500Figure 2. The average triglyceride concentrations of heart,

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