Lab Report Of Caffeine

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

Many manufactures release the caffeine content of their products publically, but not always, and new products and flavors are continuously introduced to the market. If quality checks are not performed, manufactures may alter the caffeine and benzoic acid content to suit the demands without public knowledge. To ensure the levels of caffeine and benzoic acid in products do not exceed the established safe limits and to inform the public of the amount of these compounds being consumed, various methods of analysis have been performed. Before the introduction of modern techniques, spectrophotometric methods alone were used to determine concentration of a compound in a mixture.6 The caffeine content in coffee, tea, soft drink, and energy drinks were determined using an immunoassay.7 The caffeine content in mixtures also used to have to be extracted before quantification.8

← I would repeat the experiment with the caffeine solution more times, so that the results I would get will be more

Caffeine is a wildly use drug in today’s society. Caffeine is a methylated xanthine which acts as a mild central nervous system stimulant (MS & RL, 2001). It is a stimulant which acts upon the central nervous system and increases alertness, wakefulness and restlessness and it increases the release of catecholamine from renal medullar (Fernandez, 2016; Collines, 2007). It is present in many beverages. Caffeine is found in coffee, tea, soft drinks, products containing chocolate (cocoa) and some medication (Collines, 2007). It is used as a cardiac and respiratory stimulant (Collines, 2007).Caffeine is the most frequently ingested pharmacologically active substance in the world (Collines, 2007).

Pure caffeine is a white powder, like what is found in caffeine pills, and is “a plant-based alkaloid which

Caffeine is a mild stimulant that occurs naturally in at least 63 plant species. Caffeine can be found in the leaves, stems, seeds, and roots of these various plants. Caffeine is part of the methylxanthine family. It consists of a xanthine molecule with three methyl groups attached to it. Caffeine can be found in many products like sodas, tea, and coffee, but it also occurs in several other products such as prescription medications, diuretics, and pain relievers. Caffeine’s widespread use and popularity have caused many people to view the substance as an addictive drug. Thus making caffeine the most inexpensive and readily available drug known to man. Then on the other hand there are

5.    Prepare the caffeine solution by dissolving 10g of caffeine tablets in 100ml of water in a beaker. Label the beaker ‘caffeine’. Similarly, add 10g of coffee to 100ml of water in another beaker and label it ‘coffee’.

Caffeine is a natural product that is extracted from the raw fruit of coffee plants over sixty creased alertness. Caffeine gives most people a temporary energy boost and elevates mood..species to be exact. kola nuts, cocoa, yerba maté, guarana berries, coffee beans and teas contain this. Caffeine is rapidly absorbed in about 30 to 60 minutes in our bodies after ingestion. Caffeine is defined as a drug because it stimulates the central nervous system, causing increased alertness. Caffeine gives most people a temporary energy boost and elevates mood.caffeine can also be in things like over the counter medications, and pain relievers.

Components containing caffeine were composed into stock solutions. These solutions were diluted to 1: 10 substance: mobile phase. A stock solution of caffeine was diluted 1:50. A sequence of diluted caffeine solutions were prepared for use as a standard (ppm): 1, 2, 4, and 10. Solutions of acetaminophen, acetylsalicylic acid, and Goody’s Powder were developed to differentiate chromatographic peaks observed. These solutions were subjected to HPLC for examination of the observed peak area and retention time for the set of compounds. Comparison of retention time allowed for the differentiation of peaks observed. The peak area obtained was utilized to determine the relative concentration of caffeine present in Goody’s Powder based on the relationship obtained in the standard. The content of caffeine present in Goody’s Powder by percent weight was identified.

The following procedures and materials were thought up by my partner and me, which was done on a small scale due to lack of time to perform a full fledge experiment that involved a large sample size. Our project consisted of a sample size of ten people in which each subject was given a survey to complete and a consent form to sign stating what the experiment was about. We also expressed with the up most confidence that their scores and identities within this research will be kept confidential. We articulated to each subject to not drink any caffeine four hours prior to the experiment so that we would not have any type of bias. My partner and I obtained ten cups (regular coffee cups) on day 1 and filled each cup with 200ml of water, in which five out of the ten cups had the caffeine pill ( dissolve in water before giving to subject)

In the solid-liquid extraction all water soluble components were sepreated along with the caffeine including the tannins. To further isolate the caffeine and obtain a purer product it was necessary to add sodium carbonate(Zhao et al. 208). This acidic- base reaction (tannins being the acid electrophile and sodium carbonate the base nucleophile) forms conjugate base phenolic salts. Tannins are acidic and thus have an–OH group directly bound to their aromatic ring the addition of a weaker base allows for the deprotonation of said –OH group. Then when the methylene chloride was added the tannins, now a phenolic salt, were insoluble thus resulting in the formation of two layers (organic and aqeous). The aqeuous layer now contains the tannins and chlorphyll and the organic layer the caffeine. This occurs because caffeine is more soluble in the methylene chloride and thus is separtaed into the same layer and the tannin salts and chlorophyll which are insoluble in the methylene chloride remain in the aqeous layer. The organic layer is extracted and undergeos this same washing process again in order to extract the maximum amount of product. Once enough caffeine was extracted a drying agent of anyhydrous sulfate was added to remove any of the excess water in the organic layer and placed in a hot chamber to evaporate off the methylene chloride. The remaining crystals underwent recrystalization to obtain a pure substance. (Zhao et al. 208).

1. According to the Merriam-Webster dictionary caffeine is defined as a bitter alkaloid C8H10N4O2 (not that any of you care) found especially in coffee, tea, cacao, and kola nuts and used medicinally as a stimulant and diuretic

Many of us find ourselves drinking a freshly brewed cup of coffee, an ice cold soft drink, a hot cup of tea, or an energy drink whether it be in the morning, afternoon, or in the night but one thing is that they all share a common ingredient: Caffeine

Many of us find ourselves drinking a freshly brewed cup of coffee, an ice-cold soft drink, a hot cup of tea, or an energy drink whether it be in the morning, afternoon, or in the night but one thing is that they all share a common ingredient: Caffeine

Caffeine (1,3,7-trimethylxanthine), a purine alkaloids naturally occurring in more than 60 plant species, finds greater commercial importance for its wide application in popular beverages like coffee, tea and various soft drinks. Caffeine is also one of the major agro-industrial wastes generated from the coffee and tea processing plants. Therefore, decaffeination of waste is very necessary from the point of view of environmental conservation.