Lab Report On Catalase

Hypothesis: If I place an egg in vinegar, then the outer layer of the egg is going to become slimy and look like rubber. It will also become larger and bubbles will form on and around the egg.

The human body is an incredible system that is capable of working a multitude of diverse functions. Without the help of the many different protein molecules, the human body would not be able to function properly. One major group of proteins called enzymes are mandatory for essential life. These proteins are constantly at work assembling molecules, metabolizing energy, and fighting off infections. An enzyme is a macromolecule that acts as a catalyst that speeds up a chemical reaction without being consumed by the reaction. Without these proteins, these reactions would take place too slowly to keep us alive. Essential parts in your body like vitamins and minerals cannot do any work without

Before the grape placed in the tap water was put in the tap water, the grape was very ovate, had a small dent on the side, and was soft on one end. After the grape was placed in the tap water and taken out, the grape was still ovate, but a little more plump. The grape also was still soft on one end, however the grape no longer had a dent on the side. When, osmosis occurred in the grape placed in the tap water, the water passing through the skin of the grape, acting similarly to the cell membrane of a cell, plumped the grape, pushing up the sunken skin causing the dent, therefore causing there to no longer be a dent in the grape. All four of the solutions in the experiment were hypotonic. The solutions’ hypotonicity was supported by the data showing how all four of the grapes in the different solutions gained mass because some of the water in the solutions diffused into the grapes. None of the four solutions were shown to be hypertonic by the data because none of the grapes lost mass throughout the

Salinity - All yeast spheres will be soaked in the same water, with no salt added

The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,

The tearing of the paper towel into strips; The absorption of the liquid into the paper towel strips (experiment k).

The reaction taking place is catalase breaking down hydrogen peroxide into oxygen and hydrogen. The enzyme is catalase, the substrate is hydrogen peroxide, and the products are hydrogen and oxygen. The question being tested is when we increase the yeast concentration, what will happen to the rate of the reaction. If increasing yeast concentration affects the rate of reaction, then the time it takes the disc to rise will decrease.

The procedure for this experiment was to first obtain four balloons and blow them up in order to stretch them. Then obtain and fill the four large test tubes each with thirty milliliters of warm forty degrees Celsius water and two grams of dry yeast which was weighed on a scale and scooped out by a spatula. After five milliliters of water, ten percent glucose, fructose or sucrose went into one of the four test tubes. Then parafilm was placed on top of each of the test tubes to seal them and they were swirled activating the yeast through rehydration. After swirling the film was removed and the balloons were tightly placed on the test tubes. Then finally observed the tubes build up of CO2 all the while swirling gently every fifteen minutes, recording observations.

How do different environments affect the reactivity of catalase? If we add water to the to the hydrogen peroxide, then the reaction will not be as extreme as the control group because the hydrogen peroxide will be diluted. If we add HCl to the hydrogen peroxide, then the reaction will be stronger than the control group because the acids are working together. In this experiment the independent variable was the HCl and water added into the hydrogen peroxide (10 ml each).

As a class, we wanted to find the amount of catalase activity. We achieved this by measuring the amount of oxygen produced at varying time intervals. If more oxygen is produced during a more basic pH level reaction then it would be reasonable to conclude that the pH level of a substance has a direct correlation to catalase activity. First, we worked in our lab groups in order to obtain six different sets of data. After we compiled our data into an excel spreadsheet, the mean was found for all sets of data leading toward a comparison using the t-test. Based on the evidence, the null hypothesis was rejected. I was reasonable to conclude that the more basic a substance was the more oxygen it would produce.

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

The question being answered by these experiments is: How do different conditions affect the productivity of enzymes? Enzymes are produced by cells and they act as catalysts, which means they are organic molecules that speed up chemical reactions. How effectively they work depends on the conditions they are in, such as level of temperature, pH level, substrate concentration, and salt concentration. In these experiments, Catalase will be placed in different conditions with hydrogen peroxide. How active the enzyme is in these different conditions will be measured by the amount of oxygen gas being produced. A very high increase in oxygen will indicate a high reaction rate and a low production of oxygen will show that there is a low reaction rate. On the other hand, if there is no change in the level of oxygen, it will indicate that the Catalase is denatured and is no longer functioning.

generally act as a catalase that initially bring about a chemical reaction.” Enzymes play an

Rate of Respiration in Yeast Aim: I am going to investigate the rate of respiration of yeast cells in the presence of two different sugar solutions: glucose, sucrose. I will examine the two solutions seeing which one makes the yeast respire faster. I will be able to tell which sugar solution is faster at making the yeast respire by counting the number of bubbles passed through 20cm of water after the yeast and glucose solutions have been mixed. Prediction: I predict that the glucose solution will provide the yeast with a better medium by which it will produce a faster rate of respiration.

The purpose of this lab report is to investigate the effect of substrate concentration on enzyme activity as tested with the enzyme catalase and the substrate hydrogen peroxide at several concentrations to produce oxygen. It was assumed that an increase in hydrogen peroxide concentration would decrease the amount of time the paper circle with the enzyme catalase present on it, sowing an increase in enzyme activity. Therefore it can be hypothesised that there would be an effect on catalase activity from the increase in hydrogen peroxide concentration measured in time for the paper circle to ride to the top of the solution.