Lab Report On Catalase

The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the

4.a) Describe the effect of low (pH 2) and high (pH 12) pH levels on catalase activity.

An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide.

The Effect of Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol

The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,

As a result, the enzymes were too damaged to carry out the reaction. The liver at 0°C would have slowed down the reaction rate - as was observed during the lab. The liver that acted as a catalyst at an approximate temperature of 37°C was acting at optimal temperature as the enzyme was able to perform the reaction faster due to an increased collision rate (due to temperature) between the liver enzymes and the hydrogen peroxide.

The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic. This is because the hydrogen and ionic bonds between –NH2 and –COOH groups of the polypeptides that make up the enzyme, fix the exact arrangement of the active site of an enzyme. It is crucial to be aware of how even small changes in the

The purpose of this investigation is to discover the effect of pH on the activity of catalase, an enzyme which plays the integral role of converting hydrogen peroxide into water and oxygen, and discover which pH level it will work at the most efficient rate (the optimum). The original hypothesis states that that the optimum would be at a pH is 7, due to the liver, where catalase usually resides, being neutral. The experiment consists of introducing the catalase to hydrogen peroxide, after exposure to certain solutions; hydrogen peroxide, water and hydrochloric acids, all containing the adjusted pH, and measuring the height of froth formed, an observable representation of the activity of the enzyme. The final data indicated that

An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction.

The chemical hydrogen peroxide(H₂O₂) is broken down by the enzyme catalase. Hydrogen peroxide is a byproduct formed in cellular reactions that, if not broken down, could inflict severe damage to the cell. Catalase is an enzyme that breaks down hydrogen peroxide in to water and oxygen. How efficient and strong the enzymes reaction to break down H₂O₂ determines largely on temperature and pH level. An enzyme only functions within a set pH and temperature range. Beyond that it becomes denatured, rendering it useless. The purpose of this lab is to determine at which temperature and pH level the enzyme catalase reacts best. Catalase in chicken and beef livers will be used to do the lab because enzymes still function after death as long as they are kept refrigerated at a low temperature.

It catalyses the hydrogen peroxide to oxygen and water, such as this formula: 2H2O2→O2+2H2O However, the effects of catalase is influenced by the substrate concentration. The different levels of the concentration will lead to different results of oxygen produced off the Hydrogen peroxide, Increasing the substrate concentration will increase the amount of the oxygen produced. Investigation Question: How Does increasing the concentration of Hydrogen Peroxide affect the amount of oxygen formed?

A catalase is a molecule that speeds “up the rate of a reaction without themselves being used up or permanently altered” (Audesirk & Byers 2008). They do this by lowering the activation energy required for the reaction to begin. This allows a higher proportion of molecules to move fast enough to react when the molecules collide. Enzymes are biological catalysts. They are proteins that promote specific chemical reactions. In this lab experiment, the chemical equation is: 2H2O2 2H20 + O2. Catalase, an enzyme that destroys hydrogen peroxide (H2O2), is used here to facilitate the chemical reaction from the substrate (2H2O2) to the product (2H20 + O2). This demonstrates how catalase operates in our body to destroy harmful toxins. However, the reaction

This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.

The purpose of this lab report is to investigate the effect of substrate concentration on enzyme activity as tested with the enzyme catalase and the substrate hydrogen peroxide at several concentrations to produce oxygen. It was assumed that an increase in hydrogen peroxide concentration would decrease the amount of time the paper circle with the enzyme catalase present on it, sowing an increase in enzyme activity. Therefore it can be hypothesised that there would be an effect on catalase activity from the increase in hydrogen peroxide concentration measured in time for the paper circle to ride to the top of the solution.

Enzymes, proteins that act as catalysts, are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes, the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol [2]. In the presence of oxygen, the removal of two hydrogen atoms oxidizes the compound catechol, as a result of the formation of water [2]. Oxygen is reduced by the addition of two hydrogen atoms, which also forms water, after catechol is