Lab Report On Dyes And Chemicals

2701 Words Oct 10th, 2014 11 Pages
3.1. DYES AND CHEMICALS
Six reactive azo dyes namely, Reactive Violet 5, Reactive Red 2, Reactive Orange 16, Reactive Blue 4, Reactive Black 5 and Reactive Green 19A were generous gifts from Colors India Inc. Pvt. Ltd. Ahmedabad, India. All these dyes were of industrial grade and are widely used in textile industries. Reactive Violet 5 (vinyl sulfone as a reactive group) and Reactive Red 2 (triazin as a reactive group) were used as model azo dyes in this study. All required chemicals and solvents used in the study were of analytical grade and were procured from popular chemical firms (SRL and S.D. Fine Chemicals, Mumbai, India). Biochemical and physiological test kits were obtained from Hi-media, Mumbai, India. The TLC plates were purchased from Merck India limited.
3.2. PREPARATION OF MEDIA AND REAGENTS
The Mineral Salts Medium (MSM) was prepared as per Brilon et al., (1981) with some modifications. The MSM contained of following constituents (g/l): Na2HPO4.2H2O (12.0), KH2PO4 (2.0), NH4NO3 (0.50), MgCl2.6H2O (0.10), Ca(NO3)2.4H20 (0.050), FeCl2.4H20 (0.0075) with 10 ml of trace element solution per liter. The trace element solution was prepared as follows (mg/l): ZnSO4.7H2O (10.0), MnCl2.4H2O (3.0), CoCl2.6H2O (1.0), NiCl2.6H2O (2.0), Na2MoO4.2H2O (3.0), H3BO3 (30.0), CuCl2.2H2O (1.0). Further, MSM was blended with different concentrations of Reactive Violet 5 (100 mg/l) and Reactive Red 2 (300 mg/l) and used throughout the study as a test medium and uninoculated flasks…
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