Lab Report On Laboratory Equipments And Apparatus

1483 WordsOct 2, 20176 Pages
2.1. Materials: 2.1.1. Laboratory Equipments and Apparatus: The main equipments and instruments used in this study are listed in Table (2-1). Table (2-1) Laboratory Equipments and Apparatus Equipments/ Apparatus Company Country Biohazard safety cabinet class II B Marubeni Japan Centrifuge Gemmy Taiwan PCR system or thermocycler Clever USA Cool Box Gemmy Taiwan Deep freezer-80 Marubeni Japan Digital camera Canon Japan Disposable tips Bio-Hit Finland Disposable plastic cups OEM China Distillator GFL Germany DNA extraction tubes Eppendorf Germany Dry heat block Biobasic Canada Electrophoresis apparatus Clever USA Elisa reader Lamda Germany Elisa washer Lamda Germany Eppendrof centrifuge Bio-Hit Finland Ice bath Lamda Germany…show more content…
RNA Extraction Kit RNA Extraction Kit Materials: •Lysis Solution •Washing Solution •Sorbent •RNA-eluent Green Master Mix Kit Materials: 1- DNA polymerase enzyme (Taq). 2-dNTPs (400µm dATP, 400µm d GTP, 400µm dCTP, 400µm dTTP). 3-pH 8.3. DNA Ladder Materials: 1-Ladder consist of 13 double-stranded DNA ranging in size from 100 to 1500 size bp . 2-Loading Dye which consis of (15% Ficoll, 0.03% bromophenol blue, 0.03% xylene cyanol, 0.4% orange G, 10mM Tris-HCl (pH 7.5) and 50mM EDTA). 2.2. Methods : 2.2.1 Collection of samples: 2.2.1.1 Stool samples : A total of (180) diarrhea samples and healthy sample as control (60) from children under 5 years in Hilla city the center of Babylon province were collected from patients admitted to Babylon Maternity and Pediatrics Hospital, and Al-Noor Pediatrics Hospital and many of Primary health care centers during a period extending from November 2016 to April 2017, Demographic data collection included name ,gender ,age ,weight ,immunization and feeding were collected, stool samples were collected in sterile screw cap containers for detection of rota virus and intestinal protozoa ,then storage in deep freeze (-20º C), 10 ml of blood samples were collected
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