Lab Report On Photosynthesis

Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.

The photosynthesis lab is comprised of three short experiments. These experiments showed how to understand and apply the absorbance spectrum and which colors and wavelengths correspond to visible light. In order to fully understand this absorbance spectrum and how to apply it, we initially prepared a substance comprised of acetone, a large spinach leaf and petroleum ether and measured its absorbance in the spectrophotometer. This showed us what wavelengths corresponded to the most absorption of the spinach leaf. It is understood that the least amount of absorbance should occur after 500 nm and the lower the number, the lower the absorbency. Thus, the 700 ranges is the very least amount of absorbency and the results showed us that the lowest amount of absorbance was around 740nm, which is an accurate, representation of this solution. Next, to understand what wavelengths of light drive the light reactions you can visibly see in photosynthesis, we used CMS in a variety of tubes and measured its absorbency under white light, no light, and colored filters such as red and green. We then used the chlorophyll extract we originally prepared and painted this on a chromatography strip. We measured the band distances to find out the number of pigments in the spinach leaf. These processes helped emphasize how the chloroplast pigment extract and chloroplast membrane suspension have different functional capabilities and how photosynthesis works.

In this lab, students did an experiment to figure out the amount of oxygen and carbon dioxide being produced during photosynthesis and respiration. First, they did an experiment in the light using spinach leaves. They put the spinach leaves in the chamber and turn the light on and start collecting the data in the computer. The rate of production of oxygen was high because of photosynthesis, however without light there were not photosynthesis happening, that is why there was more carbon dioxide being produced because of cellular respiration. The level of carbon dioxide increases steadily when the leaf is in the dark. The plant’s mitochondria are producing carbon dioxide gas as a by product of cellular respiration (which uses glucose

Over the past few years, DNA has become a revolutionary tool for identification purposes not only in the forensic field but also in identifying genetic defects and heritage patterns over generations. DNA, present in the nucleus of most cells, is a molecule that carries all the genetic information required for the proper development and reproduction of the cell. DNA is coiled into chromosomes and in humans, there are 46 in total. What makes DNA different from one person to another is the arrangement of nucleotides in the sequence. These can be further changed by polymorphisms. If two people are related, for example siblings, their DNA will be highly similar but differences are not completely absent.

Photosynthesis is one of the most important processes regarding living things. Photosynthesis is a process that plants undergo in order to produce food for themselves as well as giving off oxygen as a byproduct. Plants undergo photosynthesis because they are autotrophs, meaning that these organisms can gather energy from various non-living structures and store that energy in hydrocarbons (1). More specifically, most plants (including the elodea used during the lab) classify further as phototrophs. Phototrophs are organisms in which gathers the energy they use during photosynthesis from sunlight (1). The chemical equation which represents photosynthesis is as displayed below (2):

If air is removed from these spaces the change of density in the leaf. The air is return to these spaces through photosynthesis. In the lab experiment the goal is to remove the air from the disk leave and return the air into the spaces by using baking soda, instead of carbon dioxide. The rate of photosynthesis is measured in the lab by timing how many leave disks float back to the surface of the bicarbonate, water, and soap mixture.

The simple concept of photosynthesis is that it can occurs in the presence of light, which takes place in the chloroplasts of green plant cells. Photosynthesis can also be defined as the production of simple sugars from carbon dioxide and water causing the release of glucose and oxygen. The chemical equation for photosynthesis can be expressed as:

A chloroplast working solution had already been prepared by the lab professor. Seven different glass test tubes were set up and each tube was labeled with numbers 1 to 7. 4ml of the chloroplast working solution were put in each tube along with 3 drops of DCPIP in tubes labeled from 2 to 6. The contents were mixed by inverting the tubes twice. The glass tubes were placed inside colored plastic centrifuge tube (the colors for each tube were indicated in the table) and sealed with a black cap at the end. Forthwith, the test tube racks were placed under sunlight and a stopwatch was set up. All the test tubes were supposed to be brought back to the lab after the reaction in test tube complete i.e. when the color of test tube 2 changed to match the

For the purpose of the experiment , photosynthesis would require water , carbon dioxide and light. As the light produce a certain wavelength, it would show a light color for human eyes to appear. In the experiment we have tested four types of color which would have start the process of photosynthesis. It would mean that if there is light that would create a type of wavelength which would start the process of photosynthesis by starting to take carbon dioxide with water and to produce glucose and oxygen. As a way to test for those types of light we would take the spinach and place it inside a chamber , then we would take the carbon dioxide sensor and place it inside the chamber with a the type of light traveling through the chamber. The carbon

Photosynthesis is the process used by plants, algae and cyanobacteria to convert light energy from the sun into chemical energy for later use. The first stage in photosynthesis involves drawing moisture (H2O) and Carbon Dioxide from the surrounding area. The next stage in photosynthesis occurs when the light from the sun reaches the chloroplasts in the organism, for plants this is in their leaves, and for algae and cyanobacteria it happens in their antennae that collect the light. This light energy is used to convert the stored carbon and water in the organism into Glucose (C6H12O6) and Oxygen (O2). The glucose is stored in the organism whereas the oxygen becomes a byproduct of this reaction and is released into the immediate area. It is this

The process of purifying DNA plasmid to express ΔK280 human tau was done in three steps: plasmid minipreps, electrophoresis, and sequencing.

Photosynthesis is the process by which plants, some bacteria, and some protistans use the energy from sunlight to produce sugar, which cellular respiration converts into ATP (Farabee, 2010). The chemical equation for photosynthesis is:

Photosynthesis is the process where green plants use sunlight to produce water and carbon dioxide as the food and generates oxygen as a product.

Although this experiment was not an aquatic plant, it can be support for predictions made. The net formula for photosynthesis is CO2 +H2O -> CH2O +O2. Therefore, by increasing the levels of NaHCO3 (or CO2) available to an aquatic plant, the amount of oxygen produced will increase with it. To approach this experiment, an isolated testing environment will be used. The form of this being a sealed 75 ml glass tube in a 250 ml Erlenmeyer flask filled with water to stabilize any temperature changes. The aquatic plants will be evenly cut and submerged in varying concentrations of NaHCO3 solutions and placed at equal distances from an uncoloured light source. A 1 ml pipette will measure the oxygen output of the system and many trials will be made to ensure accuracy. The null hypothesis in this case will be that a higher NaHCO3 concentration will not increase/influence the oxygen production of an aquatic plant. The prediction for this lab is, if the reactants of photosynthesis are increased, namely NaHCO3, then the oxygen output of an aquatic plant (Elodea densa) will also increase with it.

Carbon dioxide is an essential component in photosynthesis. Plants intake the CO2 from it surrounds which will then react with H2O to produce C6H12O6 (glucose) and O2. This can be seen from the equation below: