Lab Report On Photosynthesis

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

This time length was chosen because longer blending periods might cause the chloroplasts to lose their functionality. While simultaneously avoiding CO2 that would cause the photosynthesis reaction to begin, four-layer cheesecloth filtered the solution so it only contained chloroplasts and not cell wall or any other organelles. Five test tubes were needed in our experiment: Tube 1, used for calibrating the spectrophotometer, contained 1 mL of buffer to neutralize the pH of the solution, 4 mL of water, and 3 mL of chloroplasts; Tube 2, the control tube, was only exposed to the fluorescent lights in the room; and three tubes that were used for the actual experiment, Tubes 3, 4, and 5, were assigned separately to each wattage variation. Tubes 2-5 were filled 1 mL of buffer, 3 mL of water, and 3 mL of chloroplasts. The calibration tube was used to set the spectrophotometer’s transmittance to a “base” or “zero” reading, making each experimental tube’s reading more accurate. The control tube held the same contents as the three experimental tubes, but it was not placed under a lamp. Instead, this tube was left alone in the fluorescent lights of the room to observe the chloroplasts’ photosynthetic behavior by themselves, without the aid of light bulbs shining on them that may increase their photosynthesis rates. Test tube racks were used to hold the tubes up in front of their designated lamps. A large Erlenmeyer flask filled

This experiment investigates the different rates at which Tagetes minuta (marigold) grow with the use of a coloured lights to determine the most suitable coloured light for the best quality of growth of marigold. “Photosynthesis is the process by which plants use the energy from sunlight to produce sugar] which is the "fuel" used by plants”. The conversion of unusable sunlight energy into usable chemical energy is linked with the actions of the green pigment chlorophyll.” (M.J. Farabee 2010).The “fuel” is stored in the form of glucose (sugar). Carbon dioxide, water, and sunlight are used to produce glucose, oxygen, and water. The process of photosynthesis can be summarized into the following chemical

Light contributes energy to photosynthesis. During photosynthesis, light provides photoautotrophs with energy. The energy is then used to convert carbon dioxide into glucose or other types of carbohydrates. But light does more than this, it also plays a role in seed germination, photomorphogenesis, and flowering. The reason for this is the quality of light. Receptors give plants the ability to differentiate wavelengths. For example, in silver birch, hydraulic conductivity is spurred by blue light.

Introduction Photosynthesis, the process of synthesizing energy from light, provides the necessary components in order to maintain flora life on earth. Implicitly, Photosynthesis plays a major role in delivering energy for other organisms that cannot synthesis their own (Education, 2016). Although this mechanism is often described as just the process of light converting into energy, it is much more complicated. Chloroplast is the platform in which Photosynthesis takes place. In this region, the light-dependent and light-independent reactions will occur (Sadava, 2013).

Photosynthesis takes place in the plant chloroplast where light, carbon dioxide and water into glucose and oxygen. Light energy is able to be captivated by a wavelength around 400nm to 700nm by photoreceptor molecule. When the light energy is maximized the molecule goes from ground state to excitation state, by releasing an electron into the adjacent molecule. One photoreceptor in green plants is chlorophyll. Chlorophyll a consume photons at 420nm and at 670nm due to its methyl group.

Aim: This report will aim to investigate the influence of wavelength on the rate of photosynthesis. The effect of different coloured light will also be explored through the rate of photosynthesis. The report will also determine the colour of light that produces the highest rate of photosynthesis.

The effect of various shading conditions on absorbance was studied using chloroplasts from Spinacia olderacea leaves. DCPIP assays can be used to assess photosynthetic activity because it is an electron acceptor that absorbs light based on the amount of electrons transferred to it during Photosystem II. Shade exposure will decrease the amount of photosynthesis the will occur, which reduces the amount of electrons transferred to DCPIP and therefore there will be less of a change in absorbance. Enriched chloroplasts were placed in conditions in which mesh was placed over light sources that created 25% shading, 50% shading, and 75% shading. Tubes of chloroplasts were also placed in no shading and completely dark conditions to serve as controls. It was hypothesized that if the percentage of shade increases, then the percent change in absorbance will decrease. Absorbance was tested using a Spectrometer at time zero, 10, 20 and 30 after the reaction began and the tubes were placed in their conditions. The hypothesis was supported in this experiment because as compared to the light, all of the shading conditions had caused a significant decrease in change in absorbance, but a plateau was observed in change in absorbance over 50% shaded. Since each shading condition did result in a significant decrease in percent change of absorbance, shading techniques can be used to prevent superfluous vegetation growth because a decrease in percent change showed diminished activity of Photosystem

There are two fundamental photosystems in chloroplasts; Photosystem I and Photosystem II. The excitation of radio wire shades in these photosystems is because of the ingestion of photons. The rate of photosynthesis can be resolved by implication by means of the blue redox marker DCPIP. At the point when lessened this pointer changes from blue to a lackluster arrangement. At the point when light is consumed by photosystem II, water is oxidized and the energized electrons are exchanged to Photosystem I for the methodology of decreasing NADP+ to NADPH. This exchange is done through the electron transport chain. DCPIP has the capacity catch the electrons that are exchanged through the electron transport chain, which will bring about the shading power of the marker answer for diminishing. The abatement in force of the pointer relates to an expanded rate of photosynthetic action.

Current studies already taken place explore the fact that certain wavelengths of light are more capable of fuelling photosynthesis (Halliwell B, 1984), demonstrating how certain wavelengths such as blue and red sections of the visible light spectrum will be absorbed by the photosynthetic organisms and then transferred along the electron transport chain, while others portions of the visible light spectrum are not absorbed such as the green section therefore, indicating why in dense forests with limiting direct light is available green light is transmitted (Lee D, 1987, Endler J, 1993).

Light is a form of energy that travels through electromagnetic waves. Light can either be absorbed, reflected, or refracted. Light is necessary in photosynthesis as it absorbed by the chloroplast and it excites electrons of the atoms in the thylakoid. The excited electrons then release energy when they go back to ground state in the electron transport chain where the energy is used to change ADP into ATP and perform photolysis which products are needed to create glucose in the Calvin cycle. Photosynthesis is important to life on earth because one product of the reaction is oxygen that is reactant in cellular respiration that is needed to be performed by most living organisms. Also plants being to perform to photosynthesis and other processes that are needed to survive are important because plants support the majority of all food chains on earth and without them life would be near to impossible to continue. Carbon dioxide is a one reactant of photosynthesis and without it the process couldn’t take place. In addition, if there’s an increase of carbon dioxide in the air or soil surrounding the plant then photosynthesis occurs at a more rapid pace. Photosynthetic pigments absorb different colors of visible light that is needed to perform photosynthesis. The goal of the first experiment was to see that carbon dioxide is absorbed during photosynthesis. The goal of the second experiment was to learn how to extract photosynthesis pigments from a leaf. The goal of the third

Photosynthesis occurs in two different processes: the light reaction and the Calvin cycle. This lab focuses on the light reaction stage of photosynthesis in which the light energy is captured by pigment molecules and utilize energy to synthesize NADPH and ATP, resulting in the production of oxygen (O₂) (Wilson, Nivillac, & Sheng, 2016, p.146). There are many factors that affect the rate of photosynthesis for accumulated O₂; however, in this laboratory experiment the

To investigate the how the photosynthetic rate of Elodea Canadensis is affected by varying its distance (cm) (±0.05) from the light source at (5cm, 15cm, 25cm, 35cm and 45cm) by counting the number of oxygen bubbles rising up the test tube per minute (min^(-1)).

The major message of this article was to identify the specific photoperiod and photosynthetic pathways and what these pathways do. Table 1. In the article shows a matrix of plant responses, photosynthetic or photoperiod pathways, sensors and light. After reading this article this illustration is useful as it shows how specific spectrums can affect different pathways and sensors which can then lead to a response such as leaf expansion and height of plant. An observation can be made that many of photoperiod pathways are involved in development such as flowering and hormone signals.