Lab Report On The Lab

759 Words Mar 1st, 2016 4 Pages
PURPOSE/OBJECTIVE:
In this laboratory, the purpose of this lab was to allow the lab students to understand the effects of the different antibiotics (CAM, Amp, and Strep) on the bacteria growths. This lab also allowed the lab students to understand the importance of staining, bright-field microscopy, spectrophotometry, and the fluorescence microscopy. The hypothesis for this lab would be that for the mixture without the antibiotic would have an increase in the absorbance rate. The mixtures with the antibiotics (CAM, Amp, or Strep) would affect the formation of new bacterial cells that would cause the absorbance rate to be lowered.

DISCUSSION/CONCLUSION

In this lab, the regular media was mixed with different antibiotics: Ampicillin (Amp), Chloramphenicol (CAM), and Strep (Streptomycin). The LB media was used as a control. The bacteria that was used in this lab was E. Coli. E. Coli was easy to be used and it have a high doubling time. We used the spectrophotometer to obtain the absorbance readings for Culture A to C. For Culture D, we got the data from another group. The starter culture in the flask, the media was cloudy, but the turbidity of the bacterial culture was still visible. With our absorbance readings for Culture A to D, we have to convert it to the number of cells by using the formula: (1 x 〖10〗^8)/absorbance_(t=o) x absorbance=# of cells. Each of the antibiotics caused different bacterial growth trends to occur. The lab students get to use the Bright-field…

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