Introduction: The Objective of the lab is to determine the mass percent of ascorbic acid in a vitamin C tablet by titration with a sodium hydroxide solution of known concentration. A titration is a procedure in which we measure the volume of a solution of a solution of known concentration that is needed to completely react with volume of NaOH analyzing. A buret is used to measure the amount of NaOH in titration and it is a piece of calibrated glassware used to accurately deliver volumes of a liquid or a solution. To determine when the neutralization reaction is complete by adding an acid-base indicator(Phenolphthalein) and it changes color at the end point. To calculate the mass percent ascorbic acid in the sample by using mass of ascirvuc acid, gmass of sample, g100%. Materials: Mortar
Pestle
Vitamin C tablet
2 Erlenmeyer flasks 250 mL
Balance
50 mL buret
Buret clamp
Support stand
250 mL beaker
Short -stem funnel
Sodium hydroxide(NaOH)
150 mL dry beaker
Stirring rod
Phenolphthalein solution Procedure: 1.Two 250 mL erlenmeyer flasks was labeled 1 and 2 and weighted
2.Vitamin C tablet was crushed into powder and transferred roughly equal into the labeled erlenmeyer flasks.
3.The mass of erlenmeyer flasks that contained the powder of vitamin c was measured.
4.The buret was mounted on the support stand by using the buret clamp.
5.”Discarded solutions” was labeled on a 250 mL beaker.
6.The stopcock of the buret was
In class, we performed a titration lab in order to determine the amount of potassium permanganate needed to titrate 1.0 g/mL of hydrogen peroxide. My team found that 42 drops of KMnO4 was needed to titrate the hydrogen peroxide solution. To find this, we first must find the amount of each reactant is in 1.0 g. To get 1 gram, 30 drops of H2O2 was needed while KMnO4 required 32 drops. In order to dilute the hydrogen peroxide, we added 2.02 g of H2O2 and 48.08 g of distilled water to get a 50.10 g solution. The next step was to find the new molarity of the peroxide.
In this laboratory exercise, we used the single celled ciliated protist Tetrahymena to be a model organism for the formation of vacuoles. For this lab, you should be able to see Tetrahymena vacuole formation through phagocytosis, and be able to calculate the rate of formation. The cilia of the organisms sweep food particles into the "mouth" of the cell, and get enclosed within the vacuoles through the process of phagocytosis. You can visualize that process through a microscope and feeding Tetrahymena India Ink, stained yeast cells, and observe vacuole formation. In order to test whether or not the concentration of India ink affects the rate of
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.
In order to test the rate of phagocytosis in tetrahymena, the tetrahymena need to ingest particles that are visible under a light microscope. The substance used in this experiment was India ink. India ink is deep black colored ink containing dispersed carbon particles. The trethymena treat the India ink as if it were food and ingest it. There were three concentrations of India ink that were fed to the eukaryotes The percent’s that were used are: 1%, 5% and 10% of India ink. After certain time intervals, the tetrahymena were fixed with a compound called 3% paraformaldehyde (PFA).On the first day six different mixtures of tetrahymena and ink concentrations were made to test which conditions would express the best rate of phagocytosis. The well fed tetrahymena were fed with 1% India ink, the another group was fed 5% India ink, the last group was fed 10% India ink. Then the starved tetrahymena were fed in the same manner as the well-fed tetrahymena. After adding the ink to the microcentrifuge, we prepared a slide for each mixture at the following time intervals 2 minutes, 5 minutes, 10 minutes, 20
2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.
Part IV 1. The precipitate was then transferred from the Büchner funnel onto a watch glass. A spatula was used to ensure that all of the aspirin had been
This is done through a change in temperature. Over time, two objects that are in direct contact will
In a test tube, 0.5mL of the sample will be added with 0.5 mL of water and shaken vigorously. Take note for its solubility by parts (0.5mL is one part). Keep adding parts of the solvent until the sample is soluble. If not, add until ten parts of the solvent and determine its solubility. To separate test tubes, water will be replaced with ethanol, chloroform, ether, and acetone as solvents. Same procedures were
In the method of continuous variations the total number of moles of reactants is kept constant for the series of measurements. Each measurement is made with a different mole ratio of reactants. A mole ratio is ratio between the amounts in moles of any two compounds involved in a chemical reaction. Mole ratios are used as conversion factors between products and reactants in many chemistry problems.
October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The
For this experiment, titrations on a weak acid, acetic acid, and a buffer were performed. Acetic acid was titrated with NaOH in order to observe the half-equivalence point as well as the equivalence point. Then, the buffer and the buffered acetic acid solution prepared faced additional titration with NaOH and HCl to evaluate the differing buffering effects following the addition of a strong acid and strong base. Finally, the buffer’s buffering capacity was calculated. If the experiment were to be repeated, it would be interesting to observe the buffering effects following a titration between a weak base and a buffer instead with greater concentrations. The change in the concentration following the preparation of buffer with a weak base and its conjugate acid would pose for an interesting experiment to observe an increase in the buffering capacity.
Titration what is that you may be asking yourself. Well a titration is a method of analyzing that will allow you determines the endpoint of a reaction and therefore the quantity of the reactants in the flask A buret is used to deliver the second reactant to the flask and an indicator or pH Meter is used to detect the endpoint of the reaction. Also just so you know a titrant is the know solution that is added and a the analyte is the unkown solution until the reactanc is complete.
Ascorbic acid, better known as Vitamin C, is a water-soluble vitamin that is important for the well being of humans and has the ability to inactivate radical compounds (Righetto and Netto, 2006). Ascorbic acid is a cofactor that is present in many enzymatic reactions that occur during biological processes such as digestion, absorption, anti-carcinogenicity, collagen formation, cataract prevention and detoxification (Vikram et al, 2004). Processing of fruits, into fruit juice, often involves a form of heat treatment which reduces the nutritional (vitamin C) content of the product (Mercali et al, 2012). Therefore, the purpose of this experiment was to investigate how prolonged exposure to heat leads to an increased degradation of ascorbic acid in mango juice. The juice was exposed to three treatments; 0, 3 and 5 minutes of being placed in a boiling water hot bath, and the concentration of ascorbic acid was determined via titration with potassium iodate.
Vitamin C, or ascorbic acid, is a well-known supplement that is essential to the human body. Vitamin C helps grow and repair body tissue, make collagen, heal wounds, and strengthen bones and teeth. Unfortunately, the body does not produce this vitamin itself, therefore it must be obtained from another source. Vitamin C is present in significant amounts in both fruits and vegetables. These foods are commonly pasteurized – a process that applies heat to destroy pathogens that cause spoilage in food. Pasteurization is great for preserving foods, but its effects on the food’s contents are important to consider. This process could affect the levels of ascorbic acid in the foods being consumed for their vitamin C content. Specifically, orange juice, one of the most popular sources of vitamin C, is going to be used to examine the effects of pasteurization on ascorbic acid levels in this experiment.