Lab Report Peroxidase

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After the experiment was completed, a trend was revealed. The trend revealed was that as the pH got closer to 7, the reaction rate increased, at a pH of 7, the reaction rate was the highest, and as the pH got farther away from 7, the reaction rate decreased. The results received from the control of pH 7 was 0 absorbance at 0 seconds, 0.114 absorbance at 30 seconds, 0.228 absorbance at 60 seconds, 0.326 absorbance at 90 seconds, 0.415 absorbance at 120 seconds, 0.495 absorbance at 150 seconds, and finally, 0.570 absorbance at 180 seconds. For the extreme pH’s, pH of 4 and pH of 10, there was no absorbance at all for the entire 180 seconds. At the pH of 6, there was an increase in the rate, and at the pH of 8, there was a decrease in the…show more content…
First off, the rate of the reaction at the pH of 7 was the highest, and this is because it was in its optimal environment (Piper 1965). If an enzyme is located in its optimal environment, or one that it prefers the most, it will be more active in that environment versus if the enzyme was located in a different environment. On the other hand, the rate of reaction for the pH of 4 and 10 was 0, or no reaction took place. The reason why this occurred is that because of the extreme pH ranges, the enzyme deactivated and can no longer complete this reaction successfully (Piper 1965). If this is examined further, it can be found that the enzyme actually denatures, or loses the shape it needs to function properly. Also, along with the preferred pH determining the activity of enzymes, the type of enzyme also determines which pH it has the most activity at. Peroxidase is an enzyme that comes from the root vegetable Brassica rapa subsp. rapa, and Peroxidase is known to affect plant growth (Kao 2001). Even though growth is not tested, it still explains that Peroxidase comes from a plant and affects plants, and plants require a neutral pH to survive. This explains why the pH of 7 has the highest rate of reaction, because the pH of 7 is neutral. It also explains the reverse, that the reaction rate at the pH of 4 and 10 is nothing, because those pH’s are
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