Lab Report : There Is A Bacteria

Method of pathogen introduction to a non-pathogenic environment includes brood stock, Aquaculture feeds and equipment. But the main method of disease introduction is due to movement of contaminated animals. Assessing of the antimicrobial use is a difficult task due to more than 200 aquatic species are

Next I performed a KOH test to further confirm that my organism was a Gram-negative species. For the KOH test, I added 3 drops of 10% potassium hydroxide (KOH) to a small drop of distilled water onto a clean microscope slide, transferred a visible clump of organism to the KOH solution using my inoculating loop. I than mixed the cells into the solution using small, circular motions for 60 seconds and then lifted up the loop to look for what appears to be a “stringing” affect which means it’s confirmed that it is gram- negative species. Next, I created a streak plate using nutrient agar so that I could see pure culture of my organism. I aseptically obtained a loop full of my organism and gently inoculated one quarter of the nutrient agar plate by running the loop back and forth across the surface. I then flame sterilized the inoculating loop, allowing it to cool for 10 seconds, and then streaked the organism from quadrant I into quadrant II using a zigzag motion technique. I repeated those steps streaking from quadrant II to quadrant III and then streaking from quadrant III to quadrant IV. Once completed, I put the streak plate in the incubator at 37° for 24-48 hours. 48 hours later, I check my streak plate and it had a lot of growth on it. I was able to determine that the organism was definitely an off white color, opaque. The IV quadrant was the quadrant that best

The Kirby-Bauer antibiotic tests indicated that the unknown is susceptible to chloramphenicol and ampicillin and resistant to penicillin G and tetracycline. The disinfectant sensitivity tests indicated that C. Freundii is resistant to all four of the disinfectants.

FISH analysis showed that the new extraction method was successful for four out of seven probes used on the sludge samples. The general probe EUB 338 showed a high density of bacteria in the seeding sludge and highly reduced densities after 24 and 28 days of operation, though still some patches with high bacterial density could be detected. In the samples from days 24 and 28 cloudlike structures were observed in all wells including negative controls, which showed high light intensity. These structures coincided with reduced hybridization ability of the EUB 338 probe (cf. figure 14 G-L) Whether the background of these structures was too high, the probe directly bound to the substances, which caused these structures or the hybridizing or fluorescent ability of the

After receiving the test results, the samples confirmed to bacteria that was in the water. The two bacteria’s where Coliform Bacteria and E.Coli. Coliform bacteria is present in the feces of all warm-blooded animals and humans. The three water samples for total coliform bacteria were 57,000 CFUs, 43,000 CFUs and 45,000 CFUs. The team of scientist was in surprise due to the high contamination level of the Coliform

Bacterial communities present in the flowback were not the exact same as the original bacteria in the freak pond. In the Struchtemeyer paper there were two different sites sampled, so there

N. fowleri is natural to the environment. There is currently no effective method for measuring N. fowleri populations or treating large bodies of freshwater for the presence of the ameba. Rarely, N. fowleri has also been found to have contaminated public water supplies. Because N. fowleri is contracted from contaminated water entering the nose, it is advised to avoid allowing potentially contaminated water into the nasal passages: do not jump or dive into warm bodies of freshwater, submerge the head in tap or faucet water, or irrigate the sinuses with tap or faucet water.

In order to detect any seasonal or spatial patterns in the prevalence and phenotypic diversity of Salmonella, E. coli, and Enterococcus species in surface waters, the phenotypic analyses of these bacteria will be conducted. Salmonella will be serotyped, E. coli will be phylo-grouped, and Enterococcus species will be determined using multiplex PCR methods 11, 12, 13. Common serotypes or species of bacteria from different seasons, years, or locations along the rivers and streams in the Upper Oconee watershed will be determined in order to find out if these serotypes and species are genetically related. E. coli will also be enumerated as an indicator of water quality.

Soil samples were collected from a construction site in North Norman and the duck pond at the University of Oklahoma. To demonstrate the microbial diversity of soil and determine the population density of bacteria, actinobacteria, and fungi, the samples were cultured in the laboratory. Serial dilutions of the samples were prepared and grown on different growth media for selective culturing of the three groups. Following incubation of the plates at 37C for two days then at 4C for five days, it was observed that the colonies on the agar plates had different morphologies. Colony counts were used to determine the original cell density of the three microbial groups. Although the calculated population densities were inconsistent because of multiple countable plates, the consistent trend was that the population density of simple bacteria was the highest in soil sample 1 and that fungi outnumbered simple bacteria and Actinobacteria in soil sample 2.

V4 Analysis also revealed the dominance of Pseudomonas among untreated SLU samples. Among other genera detected were Staphylococcus, Proteus and Bacteroides. Some of the least abundant bacteria detected were Enterobacter, Streptococcus and Corynebacterium. Treated SLU samples were dominated by Pseudomonas, Stenotrophomonas and Burkholderia, while among the least prevalent genera were Enterobacter, Proteus and Helcococcus. Bacteroides and Veillonella were only observed among untreated SLU samples, while Stenotrophomonas, Bradyrhizobium and Burkholderia were observed only among treated SLU samples (Fig 2B).

The plant management proposed to add ultraviolet disinfection to ensure the deactivation of protozoa cysts. Improving the handling of treatment plant residuals and increasing the clear well storage capacity were also identified among top problems to be addressed. Other objects covered an additional screw pump; provide corrosion control and address overall water treatment upgrades for the Plant.

Clean water is the key component to surviving life. Yet, there is some water that is contaminated that can cause disease or death if not treated right away. To determine rather the sun has a positive or negative effect on rather or not microbial grow more in an aquatic environment, three samples of water were tested from a local creek. Water that was primarily in a shaded area, where no sunlight could reach and water that was completely exposed to sunlight, with a control variable of sterilized water from the class room. Each sample was left in a stable

As most of the lakes in third pole region are pristine and oligotrophic (Wu et al., 2010), bacterial communities are unequivocally affected by the general accessibility of electron acceptors, oxidation and reduction of compounds, and the availability of organic matters from surroundings (Newton et al., 2011). Several authors revealed multiple factors playing role in the bacterial community structure in lake-water of this region. Lake primary production, pH, temperature, regional and landscape level factors (Liu et al. 2009), lake water turbidity (Sommaruga & Casamayor, 2009), and salinity (Dai et al., 2016; Liu et al., 2012;). Similarly, pH, dissolved organic carbon, and ammonia was suggested as the major environmental and chemical factors for ammonia oxidizing bacteria (Yang et al., 2016). Bacterial community in lake sediments was found to be affected by pH and geographical distance (Xiong et al., 2012). (Liu et al., 2017), recently highlighted the significant role of Carbon: Nitrogen ratio in shifting the dominant bacterial group in Kalakuli Lake.

Microbiological parameters in drinking water treatment include enteric protozoa, bacteria, virus, etc. Cryptosporidium, being a type of enteric protozoa, has caused several outbreaks in the last century. Cryptosporidiosis is the human disease caused by cryptosporidium infection, of which major symptoms are watery diarrhea, stomach pains or cramps and low fever. Between 1974 and 2001, there have been 12 outbreaks that are attributable to cryptosporidium in Canada.4 Between 1984 and 2000, 10 outbreaks were reported to be associated with cryptosporidium in drinking water in the United States.4 The most severe outbreak took place in Milwaukee in 1993 that was associated with 419,000 (estimated) cases of illnesses including watery diarrhea.3

Water quality tests on wastewater effluents from various wetlands in Nepal have shown that TSS, BOD, COD are removed by more than 95%. Similarly, coliform bacteria are also