Laboratory Testing Of Methicillin Resistant Staphylococcus Aureus

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LABORATORY TESTING OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS 36.512 Medical Bacteriology INTRODUCTION Methicillin resistant Staphylococcus aureus (MRSA) is a major cause of hospital acquired and life-threatening infections. It has also emerged recently as a cause of community-acquired infections. MRSA strains were first detected shortly after the introduction of methicillin in the early 1960s. Methicillin resistance in staphylococci is mediated by an altered penicillin-binding protein (PBP2a), which confers resistance to beta-lactam antibiotics and is encoded by the mecA gene on the mobile element, staphylococcal cassette chromosome mec (SCCmec). There are a variety of methods in diagnostic microbiology laboratories to identify…show more content…
They are considered to be an important cornerstone in preventing the spread of MRSA in health care settings. New PCR-based high throughput typing methods offer a rapid turnaround time, with lower costs and in many cases high discriminatory power. However, these are very expensive and cannot be afforded by all laboratories. Moreover, these require technical equipment limiting their use only in developed centers. DISCUSSION Typing of MRSA is an essential requirement to prevent its transmission and also to determine the precautionary and preventive measures which needs to be taken. Raman spectroscopy (SpectraCellRA) is a recently developed tool for bacterial typing. Recently, many new technologies have been introduced into clinical microbiology laboratories for the rapid identification of microorganisms such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOFMS) which are aimed at reducing the delay in providing useful information to the clinicians regarding patient care. There is an alternative, cost-effective approach which is the use of chromogenic media for screening nasal specimens such as BBL CHROMagar MRSA II (CMRSA II, BD), MRSASelect and Spectra MRSA which are used for the qualitative detection of MRSA. These chromogenic media are very selective and differential for MRSA. These media are used for the suppression of Gram-negative
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