Laccase Of Catechol

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: Laccase catalyses the oxidation of phenols by one electron oxidation, consuming molecular oxygen. The phenolic compounds form phenoxy radicals that turn into quinone intermediates in the second stage of oxidation. These quinones are very reactive and undergo non-enzymatic radical coupling reaction to form covalent bonds involving polymerisation or cross-linking of phenolic monomers. Laccase from ascomycete Myceliophthora thermophila was able to oxidize phenolic compounds such as catechol and catechin and mediate their attachment to denim surfaces. When catechol was oxidised by laccase in situ at the surface of the denim fabrics, a brown overdyed surface was obtained due to polymer deposition. Process optimization showed that 4 units per millilitre
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