Lactase Lab Report

Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is

Milk and dairy foods contain calcium which is The most common mineral in the body; calcium is needed for functions including helping blood to clot, and to build bones and teeth. Milk and dairy foods are high in protein which is essential to grow and build muscle. It is also essential for repairing and healing and repelling illnesses and fighting infections. Everything from our hair, muscles, nerves, skin and nails needs protein to build and repair itself.

Enzymes are catalysts that function to speed up reactions; for example, the enzyme sucrose speeds up the hydrolysis of sucrose, which breaks down into glucose and fructose. They speed up reactions but are not consumed by the reaction that is taking place. The most important of the enzyme is the shape as it determines which type of reaction the enzyme speeds up. Enzymes work by passing/lowering and energy barrier and in doing so; they need to bind to substrates via the active. Once they do, the reaction speeds up so much more quickly than it would without the enzyme. Coenzymes and cofactors aid the enzyme when it comes to binding with the substrate. They change the shape of the active site so the substrate can bind properly and perform its function.

In order to generate a bicyclic lactone in this experiment, a Diels-Alder adduct was produced. The bicyclic lactone to be generated was cis-1,3,3a,4,5,7a-Hexahydro-5-methyl-3-oxo-4-isobenzofuran-carboxylic Acid and was produced using a Diels-Alder reaction. The product was also analyzed quantitatively using percent yield. To prepare the Diels-Alder adduct 0.40 g of 2,4-hexadien-1-ol was added to a flask, then 5.00 mL of toluene and 0.40 g of maleic anhydride were added to the flask in that order. The mixture was warmed and stirred to induce a reaction. The reaction progress was monitored using a TLC plate with 30:70 hexane used as the mobile phase and silica gel as the stationary phase. The TLC plate revealed a new spot for the crude product, indicating the reaction had begun.

Lactose is a sugar that can be put into smaller molecules, glucose and galactose. Lactose is when you are not able to digest milk and dairy meaning that the enzyme lactase that breaks down lactose is not functioning properly. ONPG was used as a substitute for lactase because even though it is colorless it helps show enzyme activity by turning yellow. This experiment measured the absorbance ONPG when exposed to lactase within an environment of different salinity’s. The enzyme, lactase, was obtained by crushing a lactaid pill and then was added into four cuvettes. ONPG and salt solution of different concentrations were added and their levels of absorption was measured by a spectrophotometer. The results showed that higher salt concentrations have a lower level of absorption. There were 4 cuvettes and within those cuvettes that solutions within them were being tested and the results showed the more salt solution added with the lactase the lower the absorbance. The less salt solution there was a higher rate of absorbance. The data supported the hypothesis that with increasing NaCl concentration there would be a decrease in enzyme activity.

The anaerobic threshold (AT), also called the “lactate threshold,” is the exertion level between aerobic and anaerobic training. The AT is the point during exercise where your body must switch from anaerobic to aerobic metabolism. Anaerobic exercise consists of weightlifting, sprinting, and jumping. Once an athlete reaches its anaerobic threshold, lactic acid builds up in the muscles faster than it can be cleared away resulting in muscle cramps. The AT is a useful measure for deciding exercise intensity for training and racing in endurance sports. On average untrained individuals have a low AT (approximately 55 % of VolumeO2max), and elite endurance athletes have a high AT (approx. 80 - 90% of VolumeO2max). lactate threshold.(1) Studies have been conducted on finding methods of improving anaerobic threshold. Lately High-intensity interval training (HIIT) has become a popular training modality in competitive athletes, recreationally-trained individuals, and clinical populations. HIIT consists of repeated bouts of short to moderate duration exercise

Throughout our lives we have been told that milk is good for our body and helps to toughen our bones. Drinking milk started when people domesticated animals for food, which happened around 7500 years ago in the central Balkans and central Europe. By that time consuming milk was not as common as it is today. It was only farmers in some specific regions that were using cow’s milk. Milk’s market grew the mass production of meat in the 15th century. Afterwards in the 17th century, the idea of eating out and going to restaurants was born and fast food industries, started using animal’s meat for producing their meals. The rate of milk users grew with the rate of meat users and milk’s market got bigger and bigger every day. Mothers

The enzyme used in the four different experiments was lactase. Lactase is an enzyme found in the small intestine, liver, and kidneys of mammals. Lactase binds to lactose, reducing it to the simple sugars Glucose and Galactose, which can eventually be utilized as a source of energy in cells. Lactase is an important enzyme and the absence of lactase leads to lactose intolerance. According to the A.D.A.M. Medical Encyclopedia, lactose intolerance is very common in adults and is rarely dangerous. About 30 million American adults have some amount of lactose intolerance by age 20 (A.D.A.M. 2012). Some symptoms include abdominal bloating, cramps, diarrhea, gas, and nausea (A.D.A.M. 2012). These

With an important level of lactate and a prosperous protons gradient, we can see a

“Lactase is an enzyme that breaks down lactose, the sugar in milk. It is produced by the cells lining of the small intestine. Most people are born producing it, but often make less of it as they age, which causes lactose intolerance the symptoms for this include nausea, bloating, and diarrhea to name a few. This enzyme is produced

There is no doubt that milk is an important staple to the human diet especially for the health benefits it brings to growing children and teenagers. Milk provides nine essential nutrients to help develop strong bones for children and teenagers as they age into adulthood. Milk provides high quality protein, which builds and repairs muscle tissue. Vitamin B12 helps build red blood cells that carries oxygen to the lungs. Milk has calcium and vitamin D that builds strong bones in children and teens. All of these benefits shows that it is a super food and is essential to healthy growth in children and teens.

The main purpose of this lab is to identify and separate the main components of milk. To do this an understanding of the properties of these components in needed to separate them from one another. We will be separating the components with their polarity or non-polarity and the temperature at which specific components precipitate. To do this we will be using hot plates, gravity filters and vacuum filters1, water and ice baths, and blot drying.

Organisms cannot depend solely on spontaneous reactions for the production of materials because they occur slowly and are not responsive to the organism's needs (Martineau, Dean, et al, Laboratory Manual, 43). In order to speed up the reaction process, cells use enzymes as biological catalysts. Enzymes are able to speed up the reaction through lowering activation energy. Additionally, enzymes facilitate reactions without being consumed (manual,43). Each enzyme acts on a specific molecule or set of molecules referred to as the enzyme's substrate and the results of this reaction are called products (manual 43). As a result, enzymes promote a reaction so that substrates are converted into products on a faster pace (manual 43). Most enzymes are proteins whose structure is determined by its sequence of its amino acids. Enzymes are designed to function the best under physiological conditions of PH and temperature. Any change of these variables that change the conformation of the enzyme will destroy or enhance enzyme activity(manual, 43).

Lactase is an enzyme that breaks down lactose. Lactose is the sugar found in milk. It is made up of two monosaccharides: glucose and galactose. Therefore it is a disaccharide that needs to break down in order to be digested. However some people are “Lactose Intolerant” or lack lactase in their bodies. This means that they cannot consume lactose-contained food or drinks or they need help digesting it. People who are lactose intolerant must be very selective to make sure that they do not eat food or drinks with lactose. There are pills though, which are purchased by lactose intolerant people. This pill helps the people break down the sugar lactose into its smaller components.

Lactose is the carbohydrate naturally found in all kinds of milk including human milk. To digest lactose your body contains the enzyme lactase. Lactase is an enzyme supplement used to help people who are having trouble digesting milk and other dairy products. This enzyme is found in the small intestines. Lactose intolerance is the reduced ability to digest milk sugars, due to the insufficient amounts of lactase.