Lactate Lab Report

Fermentation is a metabolic pathway that produce ATP molecules under anaerobic conditions (only undergoes glycolysis), NAD+ is used directly in glycolysis to form ATP molecules, which is not as efficient as cellular respiration because only 2ATP molecules are formed during the glycolysis. One type of fermentation is alcohol fermentation, it produces pyruvate molecules made by glycolysis and the yeast will break it down to give off carbon dioxide, the reactant is glucose and the byproducts are ethanol and carbon dioxide. In this lab, the purpose is to measure whether the changes of

Four interval times (PR, RT, TP and RR) measured in seconds were recorded both with the subject at rest and after the subject had exercised. The PR and RT intervals remained virtually unchanged with the PR intervals remaining the same both before and after exercise with an interval time of 0.15 seconds, and the RT interval increase by 0.01 seconds from 0.37 at rest to 0.38 seconds after exercise. More substantial changes were noted in TP and RR intervals. The TP interval decreasing from 0.32 seconds at rest to just 0.08 seconds after exercise, a decrease of 0.24 seconds (just 25% of the resting 0.32 seconds). The RR interval decreased from 0.84 seconds at rest to 0.61 seconds seconds after exercise, a decrease of 0.23 seconds

Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is

We conducted a study, 2 subjects took their blood pressure and pulse before exercise, immediately after exercise and 5 minutes after exercise, their appearance and sweating was also noted. This table shows that before exercise both subjects had relatively normal blood pressure, immediately after exercise both subject 's blood pressure had dropped. The blood pressure was retested after 5 minutes of rest both subject 's blood pressure risen back up to a normal level. Before exercise subject A 's pulse was average, after exercise it had risen to compensate for the need of oxygen to get around the body quicker. After 5 minutes this had then returned back to a normal pace when the body had relaxed. Subject B had an average pulse before exercise, immediately after exercise it had risen, 5 minutes after exercise it had dropped again to a normal pace. the differences in pulse shows that subject A is healthier than subject B because the difference between subject A 's resting and exercise

In order to generate a bicyclic lactone in this experiment, a Diels-Alder adduct was produced. The bicyclic lactone to be generated was cis-1,3,3a,4,5,7a-Hexahydro-5-methyl-3-oxo-4-isobenzofuran-carboxylic Acid and was produced using a Diels-Alder reaction. The product was also analyzed quantitatively using percent yield. To prepare the Diels-Alder adduct 0.40 g of 2,4-hexadien-1-ol was added to a flask, then 5.00 mL of toluene and 0.40 g of maleic anhydride were added to the flask in that order. The mixture was warmed and stirred to induce a reaction. The reaction progress was monitored using a TLC plate with 30:70 hexane used as the mobile phase and silica gel as the stationary phase. The TLC plate revealed a new spot for the crude product, indicating the reaction had begun.

Lactose is a sugar that can be put into smaller molecules, glucose and galactose. Lactose is when you are not able to digest milk and dairy meaning that the enzyme lactase that breaks down lactose is not functioning properly. ONPG was used as a substitute for lactase because even though it is colorless it helps show enzyme activity by turning yellow. This experiment measured the absorbance ONPG when exposed to lactase within an environment of different salinity’s. The enzyme, lactase, was obtained by crushing a lactaid pill and then was added into four cuvettes. ONPG and salt solution of different concentrations were added and their levels of absorption was measured by a spectrophotometer. The results showed that higher salt concentrations have a lower level of absorption. There were 4 cuvettes and within those cuvettes that solutions within them were being tested and the results showed the more salt solution added with the lactase the lower the absorbance. The less salt solution there was a higher rate of absorbance. The data supported the hypothesis that with increasing NaCl concentration there would be a decrease in enzyme activity.

The anaerobic threshold (AT), also called the “lactate threshold,” is the exertion level between aerobic and anaerobic training. The AT is the point during exercise where your body must switch from anaerobic to aerobic metabolism. Anaerobic exercise consists of weightlifting, sprinting, and jumping. Once an athlete reaches its anaerobic threshold, lactic acid builds up in the muscles faster than it can be cleared away resulting in muscle cramps. The AT is a useful measure for deciding exercise intensity for training and racing in endurance sports. On average untrained individuals have a low AT (approximately 55 % of VolumeO2max), and elite endurance athletes have a high AT (approx. 80 - 90% of VolumeO2max). lactate threshold.(1) Studies have been conducted on finding methods of improving anaerobic threshold. Lately High-intensity interval training (HIIT) has become a popular training modality in competitive athletes, recreationally-trained individuals, and clinical populations. HIIT consists of repeated bouts of short to moderate duration exercise

“Enzymes are proteins that have catalytic functions” [1], “that speed up or slow down reactions”[2], “indispensable to maintenance and activity of life”[1]. They are each very specific, and will only work when a particular substrate fits in their active site. An active site is “a region on the surface of an enzyme where the substrate binds, and where the reaction occurs”[2].

To determine the effect of temperature (0- 100 °C) of the enzyme lactase using an online simulation.

'Various parameters for which contrasting observations have been reported in connection with lactase decline' (3)

The amount of glucose in the blood is expressed as millimoles per litre (mmol/l). Normally, blood glucose levels stay within narrow limits throughout the day (4 to 8mmol/l). But they are higher after meals and usually lowest in the morning Most dietary carbohydrate eventually ends up as glucose in the blood.

lactate paradox may only be a transient feature of hypoxic adaptation at altitude, disappearing in

As the intensity of exercise increased, so did the rates of the heart and breathing. After a small period of rest, the heart rate and breathing rate both decreased to a point close to their resting rate. This proved the stated hypothesis. First, the hearts average resting rate was recorded to be 76 bpm. The heart is therefore transporting oxygen and removing carbon dioxide at a reasonably steady rate via the blood. During the low intensity exercise (Slow 20) the heart rate increases to 107 bpm, which further increases to 130bpm at a higher intensity level (Fast 20). The heart therefore needs to beat faster to increase the speed at which oxygen is carried to the cells and the rate at which carbon dioxide is taken away by the blood.

Removal of lactic acid in the glycolysis system is largely impacted by blood flow and heart rate.

I predict that during exercise the heart and respiratory rate (RR) will increase depending on the intensity of exercise and the resting rates will be restored soon after exercise has stopped. I believe that the changes are caused by the increased need for oxygen and energy in muscles as they have to contract faster during exercise. When the exercise is finished the heart and ventilation rates will gradually decrease back to the resting rates as the muscles’ need for oxygen and energy will be smaller than during exercise.