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Laevis In Research

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1. X. Laevis in Research

The African Clawed Frog (X. laevis) is among the most widely used animal models to study anatomy, physiology and vertebrate development. From the 1940s to 1970s, X. laevis was used in pregnancy testing. The current standardized test for toxicity measurement of environmental pollutants; the FETAX Assay,1 was developed using X. laevis.

Recently, X. laevis has begun to be in used research investigating central nervous system (CNS) regeneration. X. laevis has also been used in ground-breaking biomedical research in developmental biology, cancer and cellular research.1

2. X. Laevis as an Animal Model

Several characteristics make X. laevis an ideal animal model. Firstly, their ability to produce large quantities of easily manipulable eggs year round,1 coupled with a long lifespan of 15-20 years1 provides scientists with a stable source of biological …show more content…

Primary research article

Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in the frog Xenopus laevis.

4.1 Purpose of Study
This study examined and compared the types and expression levels of proteins produced between regenerative stage (R-stage) and non-regenerative stage (NR-stage) X. laevis with spinal cord injury (SCI). Proteomic profiles are developed to gain essential knowledge on mechanisms controlling spinal cord regeneration and highlight reasons for its failure in mammals.
4.2 Target Groups
8 samples from R-stage tadpoles (growth stages 49-51) and NR-stage froglets (growth stage 66) were examined (Table 1).
Table 1: Breakdown of target groups examined in study.4
R-stage tadpoles (8 samples) NR-stage froglets (8 samples)
Spinal cord transection Sham operation Un-operated Control Spinal cord transection Sham operation Un-operated Control

4.3 Methodology

4.3.1 Growth of X. laevis
X. laevis embryos were grown to obtain R-stage tadpoles and NR-stage froglets. Un-operated controls were chosen from these animals.

4.3.2

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