1. X. Laevis in Research
The African Clawed Frog (X. laevis) is among the most widely used animal models to study anatomy, physiology and vertebrate development. From the 1940s to 1970s, X. laevis was used in pregnancy testing. The current standardized test for toxicity measurement of environmental pollutants; the FETAX Assay,1 was developed using X. laevis.
Recently, X. laevis has begun to be in used research investigating central nervous system (CNS) regeneration. X. laevis has also been used in ground-breaking biomedical research in developmental biology, cancer and cellular research.1
2. X. Laevis as an Animal Model
Several characteristics make X. laevis an ideal animal model. Firstly, their ability to produce large quantities of easily manipulable eggs year round,1 coupled with a long lifespan of 15-20 years1 provides scientists with a stable source of biological
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Primary research article
Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in the frog Xenopus laevis.
4.1 Purpose of Study
This study examined and compared the types and expression levels of proteins produced between regenerative stage (R-stage) and non-regenerative stage (NR-stage) X. laevis with spinal cord injury (SCI). Proteomic profiles are developed to gain essential knowledge on mechanisms controlling spinal cord regeneration and highlight reasons for its failure in mammals.
4.2 Target Groups
8 samples from R-stage tadpoles (growth stages 49-51) and NR-stage froglets (growth stage 66) were examined (Table 1).
Table 1: Breakdown of target groups examined in study.4
R-stage tadpoles (8 samples) NR-stage froglets (8 samples)
Spinal cord transection Sham operation Un-operated Control Spinal cord transection Sham operation Un-operated Control
4.3 Methodology
4.3.1 Growth of X. laevis
X. laevis embryos were grown to obtain R-stage tadpoles and NR-stage froglets. Un-operated controls were chosen from these animals.
4.3.2
The whistling tree frog does not have as many infected or dead frogs as scientists expected. Researchers theorized that the frog has evolved slightly or those who were infected as juveniles survived and became immune. Their habitat is also play a role in their
In this study, the Morgridge Institute of Research’s regenerative biology team asked when a limb is getting regenerated, what genes play a role in that process and is there a recipe that can be replicated in a different species. To observe that question, researchers observed 17 different developmental stages of axolotl embryos and found that as the axolotl grows, it goes through 3 unusual changes in gene expression that level out over time. The changes occur when the genome is first activated, during the formation of the gut and during the formation of the nervous system. With these results the scientist were given the opportunity to compare that with existing information on the axolotl limb regeneration. Also during this experiment, some pieces of the axolotl’s transcriptome or messenger RNA molecules were able to be put together so that the scientist could compare them to the transcriptomes of humans and frogs in hopes of finding common
Introduction: Many model organisms have been used in order to advance human medicine. The primary one being the lab mouse, but there are several other different species that give rise to advancements in human treatment. Planaria and axolotls have been a prominent source of how signaling mechanisms work in order to regenerate parts in eukaryotic organisms. If researchers can figure out how to turn these signaling pathways on in the conserved regeneration part of the human genome, then doctors will likely be able to use this to their advantage. This can be achieved by manipulating human signaling pathways to regenerate tissues within the heart, lungs, nervous system, and even systems with multiple tissue types like the limbs. This is where the study of axolotls comes in.
animal in the early stages of growth when it’s basic structure and organ development is forming.
Organs begin to form in organogenesis. The embryo develops into a larval stage, the tadpole. Metamorphosis then turns the tadpole into a frog.
dianae. This educational display will make the knowledge of rainforests and frogs easily accessible. In addition, the inclusion of R. temporaria will provide the visitors with extra educational information about the most common and familiar frog species in the biodiversity.
While freezing to survive the harsh low temperatures of winter is a neat trick, it does a frog no good if it remains an icicle for the rest of its life. This is where R. sylvatica’s thawing process comes into play. Thawing of R. sylvatica has been shown to begin when external environmental temperatures near -0.5 to -1.0 C. This temperature correlates to
We speculate that the southern toad is in higher abundance in the direct vicinity of water versus the dryer surrounding area due to the breeding season (February- October) and the high levels of nutrition needed (Meshaka, W. E., J.R., &
Spinal cord injuries can be extremely debilitating with significant impairment in autonomic, sensory, and motor function (Coll-Miro et al., 2016). The prevalence in Canada is on the rise with approximately 86,000 individuals suffering from such injuries as of 2010 (Noonan et al., 2012). Spinal cord injuries are generally classified as either traumatic or non-traumatic, depending on etiology (Sabapathy et al., 2015). In addition, they are subdivided into either complete or incomplete, depending on whether the spinal cord section is fully or partially damaged (Wilberger and Dupre, 2015). The latter classification has better clinical outcomes as some neurologic function is reserved (Wilberger and Dupre, 2015). Other subtypes include paraplegia and quadriplegia denoting paralysis of the lower body or all limbs, respectively (Wilberger and Dupre, 2015; Mayo Clinic Staff, 2014). The pathogenesis of spinal cord injuries is characterized by primary tissue damage due to the force of impact, followed by secondary tissue damage as a result of the inflammatory response (Sabapathy et al., 2015; Coll-Miro et al., 2015). The symptoms and severity may vary depending on the location and pathology of the contusion (Sabapathy et al., 2015). Presenting symptoms include but are not limited to numbness or pain in the extremities, loss of sensation, impaired movement or gait, abnormal reflexes, disrupted bladder or bowel function, and sexual dysfunction (Mayo Clinic Staff, 2014). Several
Model organisms are used to study embryonic development for several reasons. These organisms have shared characteristics including short life spans and generation
Showing how the Bourgeoisie exploited the Proletariat and how revolution was necessary to obtain Utopia or the removal of the state altogether.
In the beginning of our journey, it was clear that there were seven committee members that had seven different ideas of what personalizing education should look like. Visions from all areas of the board where created by our members. For example, there was talk about creating the perfect forest school, establishing a better form of democracy in education, and the debate of standardized testing belong in the public school systems. However, the main theme that we all had in common was our personal experiences with the lack of education being a priority for ourselves or a love ones in the past. It was also important that we made a difference in decreasing the academic expectations of teachers in some kind of way. The committee wanted to bring a refreshing way to the idea of partnership with teachers. This partnership would transform into a unique way of help students on their educational excursions.
The limited space within the vertebrae actually plays an important roll in spinal cord injury. Once the initial injury occurs the body, as with every other part of the body, tries to protect the injured area with swelling. But the swelling occurs within the small confines of the spinal column and causes further damage to the surrounding tissue. It has only recently been discovered how much of an impact this secondary damage has. One of the areas of crucial ongoing research is on what kind of window of opportunity medicine has in treating these types of injuries and still attaining the best recovery.
I wanted to look at two traits – Perseverance and Time Management. I collected data from two sources.
Frogs, toads, caecilians, and salamanders are the members of the class Amphibian (amphi- meaning “on both sides” and bios- meaning “life”). These members always require water for reproduction. Most Amphibians undergo metamorphosis, which is a usually degenerative pathological change in the structure of a particular body tissue. And, in the case of Amphibians, it is the changing of a tadpole into