Lion's Mane

In this experiment, 4 grams of peeled turnip was used to prepare the enzyme extract opposed to the 1 gram of turnip suggested by Fundamentals of Life Science. Along with the change to the amount of turnip used, the amount of 0.1M phosphate buffer used to prepare the enzyme extract was changed from 50mL to 30mL. The affect of temperature on enzyme activity was not

In this lab or experiment, the aim was to determine the following factors of enzymes: (1) the effects of enzymes concentration the catalytic rate or the rate of the reaction, (2) the effects of pH on a particular enzyme, an enzyme known and referred throughout this experiment as ALP (alkaline phosphate enzyme) and lastly (3) the effects of various temperatures on the reaction or catalytic rate. Throughout the experiment 8 separate cuvettes and tubes are mixed with various solutions (labeled as tables 1,3 & 4 in the apparatus/materials sections of the lab) and tested for the effects of the factors mentioned above (concentration, pH and temperature). The tubes labeled 1-4 are tested for pH with pH paper and by spectrophotometer, cuvettes 1a-4a was tested for concentration and cuvettes labeled 1b-4b was tested for temperature in four different atmospheric conditions (4ºC, 23ºC, 32ºC and 60ºC) to see how the enzyme solution was affected by the various conditions. After carrying out the procedures the results showed that the experiment followed the theory for the most part, which is that all the factors work best at its optimum level. So, the optimum pH that the enzymes reacted at was a pH of 7 (neutral), the optimum temperature that the reactions occurs with the enzymes is a temperature of 4ºC or

This experiment looked at how substrate concentration can affect enzyme activity. In this case the substrate was hydrogen peroxide and the enzyme was catalase. Pieces of meat providing the catalase were added to increasing concentrations of hydrogen peroxide in order to measure the effect of hydrogen peroxide concentrations on the enzyme’s activity. The variable measured was oxygen produced, as water would be too difficult to measure with basic equipment.

In the experiment we used Turnip, Hydrogen Peroxide, Distilled Water, and Guaiacol as my substances. On the first activity, Effect of Enzyme concentration of Reaction Rate for low enzyme concentration, we tested three concentrations of the turnip extract, and hydrogen peroxide. For the Turnip Extract I used 0.5 ml, 1.0 ml, and 2.0 ml. For hydrogen peroxide we used 0.1 ml, 0.2 ml, and 0.4 ml. We used a control to see the standard, and used a control for each enzyme concentration used. The control contains turnip extract and the color reagent, Guaiacol. We prepared my substrate tubes separately from the enzyme tubes. My substrate tube

The use of multiple test tubes and Parafilm was used for each experiment. Catechol, potato juice, pH 7 phosphate buffer, and stock potato extract 1:1 will be used to conduct the following experiments: temperature effect on enzyme activity, the effect of pH on enzyme action, the effect of enzyme concentration, and the effect of substrate concentration on enzyme activity. For the temperature effect on enzyme activity, three test tube were filled with three ml of pH 7 phosphate buffer and each test tube was labels 1.5 degrees Celsius, 20 °C, and 60 °C. The first test tube was placed in an ice-water bath, the second test tube was left at room temperature, and the third test tube was placed in approximately 60°C of warm water. After filling the test tubes with three ml of the

The role of an enzyme is to catalyse reactions within a cell. The enzyme present in a potato (Solanum Tuberosum) is catechol oxidase. In this experiment, the enzyme activity was tested under different temperature and pH conditions. The objective of this experiment was to determine the ideal conditions under which catechol oxidase catalyses reactions. In order to do this, catechol was catalyzed by catechol oxidase into benzoquinone at diverse temperatures and pH values. The enzyme was exposed to its new environment for 5 minutes before the absorbance of the catechol oxidase was measured at 420 nm using a spectrophotometer. The use of a spectrophotometer was crucial for the collection of data in this experiment. When exposed to hot and cold temperatures, some enzymes were found to denature causing the activity to decrease. Similarly, when the pH was too high or low, then the catechol oxidase enzyme experienced a significant decrease in activity. It can be concluded after completing this experiment that the optimal pH for catechol oxidase is 7 and that the prime temperature is 20º C. Due to the fact that the catechol oxidase was only tested under several different temperatures and pH values, it is always possible to get a more precise result by decreasing the increments between the test values. However, our experiment was able to produce accurate results as to the

Determining and understanding the enzyme activity in different types of Oncorhynchus mykiss muscle (heart, white and red) by comparing rate of reaction (V) of the succinate to the substrate concentration [S]

“‘Patroklos’ has fallen, and now they are fighting over his body / which is naked. Hektor of the shining helm has taken his armor,’” Nestor explains to Achilleus (18.20-21). This heartbreaking news overtakes him with such grief that he “led the thronging chant of their lamentation,” according to book 18 line 316. The poet, Homer, compares Achilleus to a great bearded lion and Hektor to a deer hunter. The lion, after leaving his cubs alone, comes back too late and realizes that they have been taken. The lion getting his cubs stolen away by a deer hunter is representative of Achilleus losing his dear friend at the hands of Hektor. Similarly, Achilleus refuses to fight and lets Patroklos fight alone in his armor, according to the beginning of book 16. Both the lion and Achilleus are anguished and angry over their losses, and each vows to avenge their loved ones. Achilleus refers to his future revenge directly after the simile, “I will not bury you till I bring to this place the armor / and the head of Hektor, since he was your great-hearted murderer (18.334-335).” The lion “quartering after the man’s trail”

These results shown from this experiment led us to conclude that enzymes work best at certain pH rates. For this particular enzyme, pH 7 worked best. When compared to high levels of pH, the lower levels worked better. The wrong level of pH can denature enzymes; therefore finding the right level is essential. The independent variable was the amount of pH, and the dependent being the rate of oxygen. The results are reliable as they are reinforced by the fact that enzymes typically work best at neutral pH

The purpose of this experiment is to learn the effects of a certain enzyme (Peroxidase) concentration, to figure out the temperature and pH effects on Peroxidase activity and the effect of an inhibitor. The procedure includes using pH5, H202, Enzyme Extract, and Guaiacol and calibrating a spectrophotometer to determine the effect of enzyme concentration. As the experiment continues, the same reagents are used with the spectrophotometer to determine the temperature and pH effects on Peroxidase activity. Lastly, to determine the effect of an inhibitor on Peroxidase, an inhibitor is added to the extract. It was found that an increase in enzyme concentration also caused an increase in the reaction rate. The reaction rate of peroxidase increases at 40oC. Peroxidase performed the best under pH5 and declined as it became more basic. The inhibitor (Hydroxy-lamine) caused a decline in the reaction rate. The significance of this experiment is to find the optimal living conditions for Peroxidase. This enzyme is vital because it gets rid of hydrogen peroxide, which is toxic to living environments.

Michael Ondaatje's In the Skin of a Lion narrates the forgotten stories of those who contributed to the building of the city Toronto, particularly immigrants and marginal individuals. In the very first page of the novel, Ondaatje stresses the concern with personal narratives and the act of storytelling: "This is the story a young girl gathers in a car during the early hours of the morning [...] She listens to the man as he picks up and brings together various corners of the story..." (4). Similar to Crossing the River, there is a framework story, that of a man telling a story to a girl, that opens and ends the novel and gives coherence to

Enzymes are high molecular weight molecules and are proteins in nature. Enzymes work as catalysts in biochemical reactions in living organisms. Enzyme Catecholase is found on in plants, animals as well as fungi and is responsible for the darkening of different fruits. In most cases enzymatic activities are influenced by a number of factors, among them is temperature, PH, enzyme concentration as well as substrate concentration (Silverthorn, 2004). In this experiment enzyme catecholase was used to investigate the effects of PH and enzyme concentration on it rate of reaction. A pH buffer was used to control the PH, potato juice was used as the substrate and water was used as a solvent.

Fungi typically have a suite of different enzymes capable of breaking down different types of plant compounds (lignin, cellulose, etc.). On the other hand, bacteria and archaea may only produce one or some of the necessary enzymes to break down these same compounds. In order for bacteria to break down those more complex compounds they have to form a consortium where each contributes a particular enzyme, each of which contributes to breaking certain bonds within those compounds. For example, cellulose breakdown requires a suite of three different enzyme systems: endocellulases break down the internal bonds, the exocellulases remove the ends of the chains, this forms cellobiose, which can be absorbed, but is further broken down by cellobiase intercellularly to create

9). (-1) represents the low concentration level and (+1) represents the high concentration level for each component. The average of results of pectinase activity and protein content are presented in (table 13).The main statistical analysis of this experiment is shown in (table 14).The main effect of each variable upon enzyme activity and protein content was estimated and represented graphically in (figure 12, 13) respectively Show that the highest pectinase activity was recorded for trial no. 6 while the lowest activity was recorded for trials No. 7 and 1.

The GSNOR activity level was measured by homogenizing 0.5g fresh leaves,roots, and stems in 0.1 M of Tris-HCl buffer. The buffer contained 0.2% Triton X-100,10% glycerol,2nM DTT,0.1mM EDTA and centrifuged at 27,000g for 25 min. The supernatants were passed through a sephadex G-25 gel. Immediately after they were passed through Amicon Ultra 3K Filters. This served as the enzyme extract. 1 ml of the volume of the mixture contained 0.5mM EDTA,0.4mM GSNO,0.2mM NADH,30 micro liiters of enzyme extract.in Tris-HCl