Lipids: Lipid and Positive Result

I. LIQUID - Identification of an Unknown Liquid: Using the physical properties of Solubility, Density, and Boiling Point.

The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test

All living things contain some form of organic macromolecules including: Lipids, proteins, carbohydrates and nucleic acids. All of these organic molecules are alike in the sense that is they are made up of bonded elements such as carbon, hydrogen, oxygen, and to smaller quantities nitrogen, phosphorus and sulfur. The macromolecules each contain large long chains of carbon and hydrogen atoms and often consists of repeating smaller molecules bonded together in a repeating pattern (polymers). To test whether a specific solid white substance is a protein is simple due to the unique chains found in the respective type of macromolecule. To test if the substance is a carbohydrate, two tests could be performed on separate samples of the object. First,

In this part of experiment, alcohol(2ml) and CH3COOH(1ml) will react to produce an ester, the ester's odor can then be compared with that of the ester bank to determine the identity of the ester. This is done by mixing the reagents in the solution with a glass stirring rod and then to further dissolve the solution, it

The primary goal of this laboratory is to correctly identify an unknown substance. To achieve this task, one may use various tests that reveal both chemical and physical properties of a substance. By comparing the results of a known substance and the unknown substance, one may eliminate alternative possibilities and more accurately predict the undisclosed compound. Furthermore, by performing these tests, data can be collected and verified regarding chemical and physical properties of the unknown. Understanding the chemical properties of a known substance aids one’s understanding of the unknown based on comparative analysis of the results of the tests.

The purpose of this lab is to test substances and to determine the physical and chemical properties of substances.

Abstract: One mixture of two unknown liquid compounds and one mixture of two unknown solid compounds were separated, isolated, purified, and characterized by boiling point. Two liquid unknowns were separated, isolated, and purified via simple distillation. Then, the process of an acid-base extraction and washing were used to separate two unknown compounds into two crude compounds: an organic acid and a neutral organic compound. Each crude compound was purified by recrystallization, resulting in a carboxylic acid (RCO2H) and a pure organic compound (RZ). The resulting mass of the pure carboxylic acid was 1.688g with a percent recovery of 31.80%, the boiling range was 244-245 °C, and its density was 2.0879g/mL. The resulting mass of the pure organic solid was 2.4902g with a percent recovery of 46.91%, the boiling range was 52.0-53.4°C, and its density was 1.5956 g/mL.

7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.

Purpose: To use indicators to test for the presence of organic compounds in certain substances.

Laboratory Tests – All laboratory tests utilize the following reference unless otherwise specified - Pagana & Pagana (2014)

1. The 1% saltwater solution was easy to create, however there was a greater amount of this solution than the alcohol. The saltwater solution worked better and had more effect on each of the colors on the strip of paper than the alcohol solution because the colors began

I believe that by testing substances with various chemicals, for example, vinegar, iodine and that will help to find out what the substance is and what is it quality.

If the IR spectra showed that it was an alcohol, Jones Test and Lucas Test can be used. If the IR spectra showed that it was either an aldehyde or ketone, we can use the DNP Test, Jones Test, and Tollens test, to determine which functional group is in the unknown. The IR Spectra can distinctively show the unknown as an alcohol, but cannot distinctively distinguish between aldehyde and ketone, because aldehyde and ketones have similar characteristic absorbances. Therefore, to distinguish between aldehyde and ketone, further chemical tests are needed. In the IR Spectra of unknown #305, it indicated that it was most likely an alcohol, due to the O-H peak at

State the chemical composition of the sterol that is present in high levels in most people who belong to the high-risk group.

Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.