Loggerpro Lab Report

The purpose of this experiment is to determine which stimuli sowbugs prefer more, moist or dark. However, to confirm that sowbugs prefer dark and moist environments we had to create an experiment to prove this. We proved sowbugs prefer dark environments by using a lamp to create a light environment and cardboard over half the petri dish to create the dark environment; the sowbugs chose the dark side. Then we proved sowbugs preferred moist environments over dry ones by creating a wet environment on one side and a dry environment on the other side of the petri dish. After our final experiment, we were able to determine that sowbugs prefer the dark stimuli over moist stimuli.

And finally into test tube 3, I pipetted 1.0 ml turnip extract and 4.0 ml of water. The contents of test tube 1 was poured into a spectrometer tube and labeled it “B” for blank. “B” tube was now inserted it into the spectrometer. An adjustment to the control knob was made to zero the absorbance reading on the spectrometer since one cannot continue the experiment if the spectrometer is not zeroed. A combination of two people and a stop watch was now needed to not only record the time of the reaction, but to mix the reagents in a precise and accurate manner. As my partner recorded the time, I quickly poured tube 3 into tube 2. I then poured tube 2 into the experiment spectrometer tube labeled “E” and inserted it into the spectrometer. A partner then recorded the absorbance reading for every 20 seconds for a total of 120 seconds. After the experiment, a brown color in the tube should be observed to indicate the reaction was carried out. Using sterile techniques, any excess liquid left was disposed

In the experiment we used Turnip, Hydrogen Peroxide, Distilled Water, and Guaiacol as my substances. On the first activity, Effect of Enzyme concentration of Reaction Rate for low enzyme concentration, we tested three concentrations of the turnip extract, and hydrogen peroxide. For the Turnip Extract I used 0.5 ml, 1.0 ml, and 2.0 ml. For hydrogen peroxide we used 0.1 ml, 0.2 ml, and 0.4 ml. We used a control to see the standard, and used a control for each enzyme concentration used. The control contains turnip extract and the color reagent, Guaiacol. We prepared my substrate tubes separately from the enzyme tubes. My substrate tube

10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted.

12.Stir then pour 2.5ml of the enzyme mixture into one of the test tubes not allowing any water from the saucepan into the test tube.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

Add to this 5 drops of pH 4 buffer solution * Measure out 2 cm³ starch solution * Start stopclock and leave for 1 minute * Measure out 1 cm³ amylase and place in second corvette * Add to this 2 cm³ distilled water *

C. Pour about one quarter of the first unknown packet into the first cup and add

2. We measured 1 mL of turnip peroxidase (the enzyme) and 3 mL of neutral buffer (pH corresponding to the test tube number i.e. pH 5 in test tube 5) with a syringe and disposed it into tubes 3, 5, 6, 7, 8, and 10

Abstract: This experiment was exploring our curiosity of sowbugs and their preferences. Our main question was about how the sowbugs natural diet can affect their preference for sodium chloride also known as table salt and glucose which is also known as sugar. The experiment involved testing how much time each sowbug spent in each substance when in a tray with both salt and sugar on opposite sides.

To test the effect of pH on enzyme production rate, adhere to the following procedure: Set up three test tubes by labelling them as pH 4, pH 7, and pH 10, and putting them in a test tube rack. Add 3 milliliters (mL) of 3% H2O2 and 3 milliliters (mL) of the pH buffer to each test tube. Squirt 2 drops of the enzyme solution into the test tube labeled pH 4. Place the test tube stopper on the opening of the test

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

test the pH of the amylase a drop of the solution should be put on pH

In addition, PH is the independent variable I am testing; therefore, its constancy is important. Variables in the experiment will need to be controlled and the following need to be kept constant * Concentration of trypsin * Amounts of reagents * Enzyme to substrate ratio

Objective: The objective of the lab is to become familiar with the Logger Pro software and Lab Pro interface in order to collect data. This lab will teach how to average constant data and find the standard deviation, as well as how to use the Statistics and Linear Fit tools. It will also introduce using Excel to create tables and graphs.