Lop of Lop

4. There are other types of reagents used to determine what type of biomolecule a substance is. For example, copper ions present in Benedict’s reagent reacts with the free end of any reducing sugars, such as glucose, when heated. Originally blue in color, these copper ions are reduced by the sugar, and produce an orange-red colored precipitate. Alternatively, iodine-potassium iodide (IKI) may also be used when working with starch. IKI contains special tri-iodine ions which interact with the coiled structure of a starch

The chart recorder was started before the concentrations of carbachol were applied to create a baseline. The first concentration of carbachol (10nM) was applied to the rat ileum by adding 50µL of the 0.01mM solution while the chart recorder was run for 30 seconds and then stopped. The Tyrode’s solution around the rat ileum was changed and left for 2 minutes between each concentration that was applied. The second concentration of carbachol (3µM) was applied to the rat ileum by adding 150µL of the 0.01mM solution. all the solutions were applied to the rat ileum using a pipette.

0.0375 mg/ml Porcine Pancreatic Amylase Solution (amylase powder in 0.9% NaCl ), Iodine Solution; each solution were pipetted into each of the 5 test tubes with 5 ml of 1% starch. Each tube contained a 1% starch solution with a different pH. All tubes were at room temperature. Room temperature was 22C. 0.2 ml of porcine pancreatic amylase solution was then pipetted into each tube. A timer was started and every 3minutes the starch / amylase mixture were pipetted from each tube and pipetted into the spot plate for every sample tube, then the iodine solution were added to a spot plate cell for each sample. Iodine reacts with starch to change from yellow to deep blue /black in the presence of starch. A lightening of the blue/ black to a brown color will occur as less starch is present. Results were reported as (+) for presence of starch in the sample or (–) for the absence of starch. After every three minute increment had passed, these same

If adding an acid or a base to liver and Potato cells then the solutions will become buffers.

A polysaccharide is when a numerous amounts of monosaccharides are joined together forming a molecule. Both glycogen and starch are polysaccharides. Glycogen is a energy storage carbohydrate found in animals. Glycogen in comparison to starch is much more highly branched. Starch is a polysaccharide that functions as a energy carbohydrate store in plants. This can be found mostly in potatoes and cereals. It is formed by the bonding together of many of glucose subunits into long chains. The actions of hydrolysis is the chemical breakdown of a compound due to the reaction of water. In order for hydrolysis to occur water must be added to the compound

To start off the experiment 25mL of water was placed into a labeled beaker and tested for the pH using pH test strips. Next the antacid tablet was placed in the solution and timed for how long it took to dissolve the tablet. Finally the temperature was measured. This same process was repeated for the acidic solution, and finally for the basic solution. After each experiment the pH, the reaction time, and the temperature of the beaker were recorded into a table. For this experiment the independent variable was the substance used (acid, neutral or base), and the dependent variable was the reaction time of each substance. The constant conditions of this experiment included the amount of substance being used, and the placing of one antacid tablet into each beaker. The experimental group was the acidic and basic solution, and the control group was the neutral

enzymes that will be used during this lab to test the ability of amylase to break down starch ,a

In part II of the lab six small glass tubes were obtained in a test tube rack. Ten drops of distilled water were then added to test tube 1, five drops to tubes 2-4, and no drops in tubes 5 and 6. Five drops of 0.1M HCl were added to test tube 5 and five drops of 0.1M NaOH to test tube 6. Five drops of enzyme were then added to all tubes except tube 1. Tube 3 was then placed in the ice bucket and tube 4 was placed in the hot bucket at 80-900C for five minutes, the remaining tubes were left in the test tube rack. After the five minutes five drops of 1% starch was added to every tube and left to sit for ten minutes. After ten minutes five drops of DNSA were then added to all the tubes. All the tubes were then taken and placed in the

2. The different concentrations of ONPG solutions and buffer solutions were incubated in the water bath at 37°C for 5 minutes.

* Temperature * Method of measuring solutions I will use equal amounts of trypsin, milk, PH, buffer and distilled water in all the experiments and will use the same measuring equipment to ensure fair representation of the effect of different PH on digestion.

This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates, then two drops of the mixture of amylase-starch is added from each temperature used, by adding iodine into the plates the mixture will show how much starch was hydrolyzed, this is used to calculate the amount of

For this experiment, we have to prepare our phosphorylase which extracted from a potato. We prepared by weighed about 250 grams of peeled potato and cut it into cubes. The extracts then blended with 100mL of 0.1M NaF. After filtered the contents into a clean 250mL centrifuge bottle, we centrifuged it for 3 minutes. Then, separated the supernatant into a centrifuge bottle, which is our phosphorylase preparation. The enzyme assay used in this experiment today is the iodine test. As the iodine reacts with starch, it will form a brown, blue or black precipitate due to the iodine ions forcing into a linear arrangement. The endpoint of the enzyme reaction indicates the presence of starch by using the iodine test to determine. The faster the endpoint is reached, the less active the phosphorylase is.

Amylase is an enzyme that is located in human saliva. It is solely accountable for breaking down starch as a way to start the breakdown of food and is one of the first steps of digestion. The time at which the enzyme starts the chemical reaction with starch is called the reaction rate. In order to study how amylase works against starch, this experiment consisted of two tests; each testing a different condition of amylase. The first test was to simply study the reaction between saliva and amylase and note the reaction rates. The second test was to see if increasing the pH would decrease the reaction rate or halt it all together. Saliva was collected, diluted, and tested for reactions between starch and amylase. Another sample of saliva was collected, diluted, and had its pH increased and tested for reaction rate. The findings after the experiment was conducted aligned with the original hypothesis. The change in pH did show a significant decrease in the reaction rate.

In the final experiment we filled a dialysis bag with starch solution and tied off both ends of the bag so that it is water tight. We then filled a separate bag with sodium chloride and submerge both dialysis bags in two beakers of distilled water. We allowed the bags to sit in the water for 10 minutes. We then put silver nitrate into the water that held the dialysis bag filled with sodium chloride and recorded any changes in the water. We then added iodine to the water that held the dialysis bag of starch and observed any changes in the water.

Add 5mL of gastric juices (contains both pepsin solution and HCl) to test tube 3