Before starting the experiment we believed that the high light and high nutrient treatment would cause bacteria to grow more quickly because it had all resources available for the bacteria to be successful in having a high growth rate and doubling time. This sample would experience the least amount of limitations. With that being said, the expected treatment with the lowest growth and doubling rate would have to be the low light and low nutrient treatment. It was seen that in comparison to low and high nutrients, there was a significant difference in growth rate when using a high nutrient sample. When comparing high/low light using a low nutrient sample, there was no significant difference between the data (Table 4). This may mean that light was not a major contributing factor when in a low nutrient environment. When looking at effects of nutrients, we ran comparisons with the same light intensity. We saw that there was no significant difference between low and high nutrients in neither low or high light (Table 5). This does not fully support our hypothesis because the comparison of highlight/low nutrient v. high light/ high nutrients …show more content…
Since phytoplankton live in a variety of environments, future studies can factor in comparisons of different species of phytoplankton and see how treatments such as these affect each one. Continuing using controlled studies is important. Using actual physical environments can pose problems. These problems could stem from unpredictable weather, oceanographic conditions, and hard to define taxonomy of these phytoplankton (Cloern and Nichols 1985). If studies continue to us the phytoplankton chlorophyte, Tetraselmis spp. , you can add in the factor of salinity into the experiment. This species is known to withstand many different salinity levels (Fabregas et. al.1984) and seeing how that might affect data would be an interesting path future studies can
For an existing habitat, an increase in salt levels will begin to show an effect on the abundance and diversity in species of zooplankton. This will exhibit a detrimental effect on the daphnia from the limited osmoregulatory capabilities of the organisms. This in turn can pose a risk to the level feeding rates and survival (Heine-Fuster, Vega-Retter, Sabat & Ramos-Jiliberto,
In this experiment we are testing the effect of fertilizer on the speed of plant growth. We prepared a 4 quad cell, 1 control group and 3 experimental groups. So, we had one with no fertilizer, one with three seeds of fertilizer, one with six seeds of fertilizer, and lastly, one with nine seeds of fertilizer. The plants that we grew were called Wisconsin Fast Plants, members of the crucifer family. These plants are small and easy to grow, but for optimal growth they require continuous fertilizer, water, fluorescent light, and temperature between 18 degrees Celsius and 26 degrees Celsius 24 hours a day. Fertilizers are substances that are put into soils to increase the growth of the plant. There are two different types of fertilizers, synthetic
The growth and survival of a plant depends on the reactions that occur internally called photosynthesis. Photosynthesis is a reaction that captures the sun’s energy and converts it, water, and carbon dioxide into glucose, with a byproduct of oxygen. Glucose is a sugar that provides energy to allow for a plant to grow and live. This experiment is to test how photosynthesis can be sped up with a home solution. The variable being changed in this experiment is the solution that the plant is being given. In this instance, some of the plants will be given Gatorade, rather than water. The question being asked is, How well will a solution found in the home affect plant growth?
As shown in the figure above, it is evident that V.natriegens grew faster when the Brain Heart Infusion (BHI) broth contained 250mM NaCl. The # of bacterial cells at each time point was measured following the equation given in the “How to generate a bacterial growth curve” supplemental material posted on D2L. (2) The data was then recorded in the table listed above. A growth curve graph was constructed using the data above which illustrated the differences between each of the different BHI mixtures. The graph was then used to determine the generation time of V.natriegens for each different environmental condition. In order to calculate the generation time (g) the mean growth rate (k) must be calculated. The formula to do this is posted in the supplemental material “How to generate a bacterial growth curve” on D2L. The k value calculated for each condition goes as follows:
Therefore some suggestions for improvement in future test include: conducting tests whilst setting controls such as time of the day and condition over a period of time to get the most accurate answers possible. Eg. Dissolved oxygen rates can vary over the period of the day. Another improvement would be following the methodology more strictly to reduce any margin for error, clearly apparent in our phosphate test. But despite our best efforts, there were some events that could not be avoided such as an oil spill in the creek that would have impacted on the macro-invertebrates results we tested after this
After collecting all eight samples, they were then placed in the Petri dishes. The Petri dishes were both placed in an incubator. The temperature at that time was 80 degrees Fahrenheit. The samples remained in the incubator for over 24 hrs, by which time, quite a lot of growth was formed.
We hypothesized that at Miracle-Gro concentration 1, this solution will be the most effective towards the rate of growth because at pure solution, the concentration is so high that the effect will occur sooner than those of lower concentrations, and speed up the life span to expiry of all the plants in the single solution concentration. Because we accept as true, that pure concentration will have the highest rate of growth, we can eliminate this concentration from our second question. We have come up with the presumption that at the concentration of 0.1 solution will allow the plants to be the most successful for the longest period because concentrations less than 0.1 would not appear to have any effect. There would be limiting nutrients within the solution and therefore would have a yield due to the lack of Miracle-Gro
Assignment: As a Registered Dietitian, you have been invited to a “Lunch and Learn” program at a very large law firm. The firm must make a decision about which company they will select for a year long beverage contract. The lawyers have three choices: water, Gatorade or Powerade. Out of the 55 attorneys that will be attending your presentation, 32 of them workout regularly, about 60 minutes, five days per week.
This lab was conducted to see what happens when black tea and water are used to enhance the growth of a radish plant. The hypothesis was that the plants that were given the black tea and water mixture were going to grow faster than the control plant (Devonte). The hypothesis was partially correct because all the plants grew substantially large closer to the end of the lab. Plant 2 (Bravo) started to wither away and die because it was over fed, and eventually drowned in water. The other two plants grew at a consistent pace and developed into healthy mature plants. To sum up, the lab that was conducted was successful in the sense that two out of the four plants grew rapidly without the use of enhancements or
Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object
coli was seen to have a faster growth rate in the 0.5% garlic extract dilution. The growth rate of the 0.5% garlic extract was 1.358 generations per hour. In contrast, the growth rate for 0% garlic extract was 0.882 generations per hour which was the smallest out of the four garlic extracts. In between the 0% and 0.5% garlic extract, the 0.1% and 0.25% garlic extract had growth rates of 0.907 generations per hour and 0.816 generations per hour respectively. With 0.5% garlic had the fastest growing rate, a E. coli generation forms as fast as 44.181 minutes. The 0% garlic extract developed a generation with a time generation of 68.028 minutes, and the 0.1% and 0.25% garlic extracts had time generations of 54.410 minutes and 48.912 minutes respectively. E. coli in the 0.5% garlic extract dilution had grown quickly and surpassed the other 3 dilutions by hour 8 with an OD600 of 1.735 and remained the second highest at hour 25 with an OD600 of
Alyssa E. Beck conducted an experiment in which her purpose was to observe the effects of short term ultraviolet light exposure on bacteria. In this experiment, she placed and labeled bacteria samples onto plates and exposed the bacteria to ultraviolet light for two, five, and thirty minutes. For each sample, half of the plate was exposed to the ultraviolet light while half was not, serving as the control group. The results of the experiment showed the bacteria not exposed to the uv light grew into distinct colonies while the none of the exposed bacteria survived after the time periods (Beck
This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density
We can measure how certain variables like temperature, ph and oxygen levels affect on biological processes such a growth rate, reproduction and mortality. The organism we choose to study can be from different trophic levels to see how these changes affect the ecosystem as a whole and the food web. We can take measurements of these factors where they are high and see how they affect the organism. Additionally, we can test these variables individually in close systems to see what type of effects it has. In a video we watched in class we saw how rising pH could affect corals killing them off with coral bleaching and if acidic enough the coral itself would erode away because it was under the saturated state. Understanding these effects is essential to preserving our ocean and its
Plankton are aquatic organisms that drift throughout their environment as they posses no true motor capabilities. Within their respective aquatic habitats plankton form the productive basis of their ecosystem and are divided into two subcategories: zooplankton and phytoplankton, the later will be the primary focus of this paper. Phytoplankton, as well as other photosynthetic organisms poses pigments called chlorophyll. These pigments allow phytoplankton to convert carbon dioxide and water to oxygen and sugar, which provides the phytoplankton with energy. Due to the fact that all phytoplankton posses chlorophyll scientists have developed methods that use chlorophyll testing in order to understand more about phytoplankton as a whole. Phytoplankton posses different types of chlorophyll but scientists usually sample chlorophyll-a as it is the most abundant form (YSI Environmental, Cullen 1982, Santos 2003). Several different methods have been developed to analyze the concentration of chlorophyll-a.