M & M Color Lab

7. Tape the strip to a pencil and rest the pencil on top of the jar so that the strip hangs into the jar. The goal is to have the end of the chromatography strip just touching the surface of the solvent solution, with the colored dots above the surface of the liquid. Make sure that the colored spots do not come in direct contact with the liquid in the bottom of the glass.

The concentrations and absorbances of the red and blue dyes were used to find the concentration of the purple dyes. From the graph of the blue dye, the linear equation for absorbance was y = mx + b. From that formula came the equation y = 7.915 x 104 (x) + 0.02489, where y represents absorbance, m is slope, x is concentration/molarity, and b is the constant/y-intercept. The same set up was performed for the red dye, but the equation produced was y = 1.045 x 104 (x) +.001298. The equations found when graphing absorbance vs. concentration were used to find the concentration of the purple dyes. The absorbance for purple dye 3 on the red wavelength of 470 nm equaled 0.149 and 0.818 for the blue wavelength of 635 nm. For purple dye 1

Answer: Once the chromatogram has been completed and is ready to be measured and calculated, on the plate that was used to perform the chromatogram you should see where the red and blue have completely separated. The red food coloring dye should be lower on the plate than the blue food coloring dye.

I say that because it started out as a dark green then turned into a light colored green. The greater change in this experiment for me and my partner was that volume had a greater change. In the data it shows that the volume of the gummy bear is 7.77cm3 as the difference in day 1 and 2, compared to the mass it is only 4.12 grams which is less than 7.77. There was a change in density, the difference was .841 and on the first day it was .905 and on the second day it was .64g/cm3. When I had to share a digital scale I remembered my mass and when I saw there mass added to our It was only .95 added to our 6.72

Before the food lab experiment where we tested milk, crackers, bananas, and egg whites for simple sugars, starch, and proteins I expected to learn about what foods contained any of the three possibilities, if not all of them. The lab provided my group and I information afterwards that can further my education into macromolecules and biology as a whole subject. My group and I predicted that milk will contain simple sugars, crackers will have simple sugars and starch, bananas will have proteins, and the egg whites will contain proteins. If milk does contain simple sugars, during this lab when my group and I test, then the milk should turn from a blue color to a green, yellow, orange, or red color when heated. Crackers should have the same outcome as milk if it does contain simple sugars, but if crackers also have starch the crackers should then turn into a blackish color. We predicted that bananas contain protein and if so, then the solution should turn from a light blue to either a dark blue or a purple. Finally, egg whites are what my group and I finally predicted to contain proteins. If our hypothesis is correct, the solution should do the same as it does for the bananas.

Every cell transports materials in and out throught something called a membrane. There are many different methods of transport in the cell Saccharomyces cerevisiae (Serrano, 1977) We want to know does adding higher concentrations of azide more effectively block dye transport? We tested the transport of dye in yeast cells with a metabolic inhibitor. When we did this we showed no difference in the absorbance between different azide solutions, and our control. From this we concluded that azide has no effect on the transport through a yeast cell membrane.

1. Paper Chromatography is a method used for the separation of colors which are also referred to as colored chemicals/substances or pigments. This method is used for experiments, to identify coloring agents and to separate out a compound into its various components.

Results: For part one of Lab 3a, Day one, the beginning unknown mineral was a blue-green color. The mineral was charcoal colored after the roasting process. Roasting caused the mineral to lose mass. The lost mass was due to release of gas.

At first I was going to do “What happens when different M&M’s hit different substances/liquids,” I had to change the Question to What was the first M&M to react with different substances/liquids,” and it ended up amazing. I wish I could have done all of them doing the race, and it would have been more accurate. One question I have is Do M&M’s have a brown coating under the white coating? That is my

Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance, followed by chlorophyll a. Chlorophyll b is the most polar; therefore, it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is

I observed how the red food dye color only went in the first three paper towels and not the rest of the paper towels.

Some pigments move further than others because of the size of their particles. Smaller, lighter particles will move through the filter paper more swiftly than larger, heavier particles. This is true because smaller, lighter particles are more soluble in solvent, thus allowing them to move further. In addition, a pigments affinity for paper will affect its distance travelled. The more polar a substance is, the greater attractiveness it will have to paper through hydrogen bonding (Jeffrey & Saenger 149). Therefore, the pigments particle size, solubility, and attractiveness to paper will determine the distance it moves.

It is possible to separate these pigments from each other using a technique called paper chromatography. In this process, plant tissue extract is applied to a piece of chromatography paper. “A solvent is allowed to travel up the paper, and if the pigment is soluble in the solvent, it will be carried along with it.” (Benya, 2009) Different pigments have different affinities for the solvents or polarity and will travel at different rates. Chlorophyll, anthyocyanins, and carotenoids are typically non-polar.

The next step was to place the strip of chromatography paper on a paper towel. Then dip a capillary tube into the plant pigment extract (spinach pigment extract) provided by the teacher. The tube will fill on its own. We applied the extract to the pencil line on the paper, blew the strip dry, and repeated it three to four times until the line on the paper is a dark

As the solvent moves past the spotted mixture, two opposing forces created by the solvent and the adsorbent influence the mixture. Each component can either dissolve in the mobile solvent or remain adsorbed to the stationary adsorbent. This process generates an equilibrium, as some components are adsorbed and others are dissolved and transported with the solvent until they are readsorbed further along the plate. The different tendencies of each component to comply with the subjected forces may result in a successfully separated mixture on the plate.