Mass Spectrometry of Protein Analysis

Colorimetric assay is a process determining the concentration of a chemical element or compound in a solution

We can use this to do mathematical conversions to determine mass, volume, and number of atoms or molecules in a given substance. For example let’s check out this video to convert moles methane (CH4) to grams of methane. http://www.sciencegeek.net/Chemistry/Video/Unit4/GMV2.shtml

3. Electrons are small and are negatively charged (-) with a mass of almost 0 amu..

10. The sum of the oxidation numbers of all atoms in a polyatomic ion is equals to the charge of the ion.

the ions of SO42-, CO32-, Cl-, and I-,identify them separately, and use the observation to identify the unknown.

AAS has contributed to the understanding of elements having different absorption emission spectra due to their difference in energy levels. In the absorption spectrum, the absorbed light are shown as black gaps. As the number of electrons increase, the number of spectral lines also increase. Hence, by measuring the absorption of light, the concentration of the element within a sample can be determined. By knowing the concentrations of an element, scientists are now aware that even the smallest amount can make a significant impact towards the biological system. Therefore, scientists have brainstormed ways to monitor the use of chemicals in the

Using the direct weighing and weighing by difference methods to find weight and mass of chemicals and compounds.

Analysis mode: The procedure is done to estimate different types of molecules and their individual quantities in the mixture using the help of a detector.

The start of the experiment consisted of filling up four beakers with de-ionized water to 150 ml. After the beakers were filled to the appropriate amounts they were then labeled with the

The main objective of this experiment is to carry out qualitative analysis to identify metal cations in unknown solution 1.

     After the column the separated compounds enter the detector, which measures a physical or chemical property of each, now relatively pure, compound and creates a proportional electronic signal. By calibrating with a standard mixture of known compounds, the nature of the compound in the

The purpose is to separate the elements from each other in a mixture and to recognize if there are any associated physical changes of those elements. Moreover, it’s intended to recognize the percentage of error of each element’s weight after the separation compared to its reference percentage in the mixture

This distinct property of compounds exploited by the MS, can be used to identify it. Hence, the GC and MS components together can be used for the identification of compounds whereas a stand-alone GC or MS machine will not. It is extremely unlikely that two different compounds will behave in the same way in both a GC and a MS. Therefore, a mass spectrum along with a characteristic retention time, typically helps identify a compounds with a lower possibility of

of being able to analyze multiple samples in a short amount of time. The most efficient way of determining concentration is to prepare a set of standard solutions of known

Going from solution to ions using electrospray is an interesting process. The analyte or sample that is being