Materials And Methods Of Chemicals

1058 Words Sep 17th, 2016 5 Pages
Materials and methods
Chemicals used:
Fipronil (Insecto SC 5%) is a product of BASF Company and manufactured by Sinochem Group- Ningbo Technical Co. Ltd, China. Zinc was obtained (in the form of zinc sulphate heptahydrate) from October Pharma, Egypt. The kits used for the following biochemical assays were purchased from Biodiagnostic Company, Dokki, Giza, Egypt: superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GPx, EC 1.11.1.9), glutathione-s-transferase (GST, EC 2.5.1.13), glutathione reduced (GSH), lipid peroxidation (LPO) and total protein. All other chemicals were obtained from reputed companies.
Animals and experimental design
Twenty male albino rats, weighing 90 ±10 g were used in this study. The animals were obtained from the animal house of the National Organization for Drug Control and Research (NODCAR), Dokki, Giza, Egypt. They were housed under normal environmental conditions of temperature and humidity and allowed to adapt to the new environment for two weeks before starting the experiment. Animal rooms (23±2 Cº) were maintained on a 12:12h light/dark photoperiod. Animals were provided with food with free access standard pellet diet and water ad libitum. All animal procedures were conducted according to accepted standards of animal care following NODCAR Guidelines. Rats were randomly divided into four groups, five rats in each group. Control group, rats received drinking water. Zinc group, rats received zinc at…
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