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Membrane Potential Lab Report

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The findings of this experiment reinforced the hypothesis that the resting membrane potential is most influenced by the ion potassium. We were able to deduce this through the collection of a multitude of intracellular and extracellular recordings, such as the one shown below in Figure 1. This shows how this experiment was able to record every single resting membrane potential in all three different muscle groups under all six solutions. The fact that potassium is indeed the most influential determinant of crayfish resting membrane potential can be seen through the analysis of Figure 2. This table shows that the obtained resting membrane potential follows the same trajectory as the Nernst calculated one. The initial concentration elicited a resting membrane potential that was significantly more positive than…show more content…
Hence, this would allow for an influx of sodium into the cell down its electrochemical gradient. It would also allow for the flow of potassium outward, as it has a 140mM concentration inside the cell and wants to shift down its concentration gradient to 5.4mM. Therefore, this great driving force for the influx of sodium and efflux of potassium helps to explain the findings at this point. As Table 1 shows, the findings within this figure are statistically supported. The fact that there is not significant difference between the findings of this experiment and the calculated Nernst at the 10, 20 and 40mM of potassium is an indicator that sodium is the largest determinant of the resting membrane potential. However, some findings defy the expectations, as the last two concentrations elicit a resting membrane potential significantly more negative than expected. This can be explained by the fact that at this point, each muscle at their respective muscle groups has been protruded many
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