Metabolic Reaction Lab Report Essay

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BIO 211 Lab Section 11
February 15, 2012
Effects of Temperature on Enzymatic Activity
Temperature is a measure of kinetic energy. As this movement increases, collision rate and intensity, and therefore reaction rates, increase. This experiment was conducted to determine if there is a minimum temperature that increase kinetic energy and denature enzymes to slow enzymatic reactions or fail to catalyze them. The experimental results indicate an increase in temperature will increase reaction rates until proteins denature.

Enzymes combine with reactant molecules (substrate) and bind them closely to one another. The three-dimensional shape of the enzyme molecule must be complementary to the shape of the substrate.
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al., 2007).
To test this hypothesis, four test tubes filled with 9mL of a solution of baker’s yeast, which are exposed to different temperatures. In the four beakers of water, all with a different degree of temperature, the test tubes are placed. Then, a small piece of filter paper soaked in hydrogen peroxide is dropped in the test tubes. The time that it takes from dropping the filter paper into the test tubes until the paper floats at the top is recorded.
Four test tubes were filled with 9 mL of a solution of baker’s yeast. Of the four test tubes, one of them was placed in a beaker filled with water at room temperature, another in a beaker of water at 37 degrees Celsius, one at 65 degrees Celsius, and the last one was placed in a Styrofoam container with ice at 0 degrees Celsius. Once the test tubes reached their desired temperature, they were pulled out of their beakers. Four pieces of filter paper were cut and soaked in a hydrogen peroxide enzyme solution. Once soaked, each of the filter papers was dropped into the test tubes. When the papers reached the bottom of the tubes, they were placed back into the different temperature beakers. The clock was then started to see how long it would take for the filter paper to reach the surface of the tube. The reaction rate (dependent variable) was measured in seconds that it took for the filter paper to reach

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