Microbes : Antagonistic Microorganisms : Bacillus Amyloliquefaciens Mb101 ( Ba ) Essay

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for

The first result of importance was the result of the Gram stain. The observations of the unknown bacteria from the slant culture after Gram staining showed that the unknown bacteria were Gram negative bacilli (Image 1). After determining the unknown bacteria was Gram negative, an oxidase test was conducted on a sample from the slant culture. The cotton swap with the sample of bacteria did not change color when the oxidase reagent was applied, thus providing a negative result. With a negative oxidase test, further tests were conducted to determine various characteristics of the unknown bacteria. A MR-VP broth was inoculated with a sample from a slant culture of unknown bacteria. After incubation, the methyl red reagent was added to the broth, and the broth turned red, providing a positive result (Image 2). An EMB agar streak plate was inoculated with a sample from a slant culture of the unknown bacteria, and after incubation, growth was found on the plate, providing a positive result (Image 3). A Citrate agar slant was inoculated, and after incubation, growth was found on the media, providing a positive result (Image 4). A Urea agar slant was inoculated, and after incubation, the agar had changed from a peach color to a bright pink color, providing a positive result (Image 5). Using the flowchart (Figure 1) developed from the Table of Expected Results, the lab partners started at the oxidase test. Given the negative result of the oxidase test, the flowchart is

The antibiotics will have different zones of inhibition on different bacteria. Also, a range of concentrations of the nutrients in the bacteria will change the bacterial growth.

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and sucrose fermentation, Kugler/iron agar, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, bile esculin,

During the purification section of this lab, the LB/amp/ara agar plate was examined for well-isolated green colonies and the LB/amp plate was observed for white colonies with space between each other. These colonies were circled on the outside of the plates using a marker. Next, two 15 milliliter culture tubes containing 2 milliliters of nutrient growth media were obtained and labeled “+” and “-“. Using a new inoculation tube, the circled colonies from each plate were scooped out and immersed in their respective culture tubes. Once the bacteria was mixed into the solution, the tubes were sealed and placed horizontally into the 32⁰ incubator for 24 hours.

The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function.

As mentioned in The Littlest Farmhands, the fungus-like organism called Phytophthora infestans is known to cause potato blight and other crop diseases which leads to famines. There are many other fungi and bacteria that cause the roots and leaves of the plant to decay. On the other hand, microbial groups such as rhizobiome, have been discovered to help fight off crop pests and diseases. Other microbes have been found to help plants thrive in dry or salty environments; this will be beneficial when the world needs to adapt to climate change (Jop de Vrieze, pg 681). There is another beneficial microorganism called mycorrhizae, which forms fungal filaments at the base of the plant’s roots. These filaments carry nutrients and water to the plant cells for survival, and they let different crops communicate with one another to form a defense system in the event of a pathogenic attack. I strongly believe farmers should switch to the more natural way of taking care of their

I believe that the test that we performed was not effective in distinguishing between bacteriostatic and bactericidal agents because there is no distinguishing characteristics between the zone of inhibition of a bacteriostatic agent and a bactericidal agent.

: In the field of microbiology, there are times when a sample will contain more than one species of bacteria. The goal is to separate each bacterium and culture them independently from one another to identify them. This was the objective of this lab. Each stock contained two unknown bacterium, and the possible unknowns were Eserichia coli, Enterobacter aerogenes, Enterobacter cloacae, Serratia marcescens, Proteus vulgaris, Klebsiella pneumoniae, Shigella flexneri, Shigella sonnei, and Salmonella enterica. The tests available were MacConkey agar, Endo agar, Hektoen Enteric agar, Tryptone Soya Agar (TSA), carbohydrate sugar broths, Triple Sugar Iron (TSI) agar, decarboxylase broths (arginine, lysine, and ornithine), Simmon’s Citrate Agar, urease

In our world we are surrounded by microorganisms, some are harmless, many are beneficial; they are a part of our normal flora on our skin and in our intestines, providing a barrier against microorganisms that may not be so harmless. It is important, however, to distinguish which microorganisms are potentially pathogenic from those that are not, and when someone does get ill to determine what the organism is so appropriate treatment can be determined.

Antibiotics are important for the treatment of bacterial infections, and it is therefore important to know how different antibiotic works in different contexts. Different kinds of bacteria are affected to different degrees by different antibiotics, and it may therefore be useful to know which antibiotic that attacks the bacteria. The purpose of this report is to analyze how antibiotics including Chloramphenicol and Ampicillin affect the growth rate of bacteria Escherichia coli and Bacillus subtilis. Which antibiotic is the most effective or least effective in inhibiting the growth rate of these two bacteria respectively?

Biological agents, coming in a variety of forms, have unique abilities amongst themselves and all categories of environmental health agents. Bacteria holds the ability to

These mutations, no matter what process that has led to their occurrence, block the action of antibiotics by interfering with their mechanism of action (1). Currently, antibiotics attack bacteria through one of two mechanisms. In both mechanisms the antibiotic enters the microbe and interferes with production of the components needed to form new bacterial cells. Some antibiotics act on the cell membrane, causing increased permeability and leakage of cell contents. Other antibiotics interfere with protein synthesis in cells. They block one or more of the steps involved in the transformation of nucleic acids into proteins.

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to