Microbial Diversity And Bioremediation Of Toxic Heavy Metals From Ash Dyke Of Power Plants Of Chhattisgarh
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MICROBIAL DIVERSITY IN BIOREMEDIATION OF TOXIC HEAVY METALS FROM THE ASH DYKE OF POWER PLANTS OF CHHATTISGARH
SONALI BANERJEE*1, RAGINI GOTHALWAL2, PANKAJ SAHU3 & SHWETA SAO4
PhD SCHOLAR1 & HEAD4, DEPARTMENT OF LIFE SCIENCE, DR C. V. RAMAN UNIVERSITY, BILASPUR (C.G), INDIA
ASSOCIATE PROFESSOR, BOTANY, RANI DURGAVATI VISHWAVIDYALAYA, JABALPUR (M.P)
HEAD3, DEPARTMENT OF BIOTECHNOLOGY, BARKATULLAH UNIVERSITY, BHOPAL (M.P), INDIA
Email: sonalibanerjee.biotech@gmail.com
Ph: +919755518186,
Abstract
Approximately four varieties of bacteria has been isolated from the ash dyke sample of four power plants of Chhattisgarh. Total 6 isolates were found to be Pseudomonas sp, 12 isolates are Bacillus sp., 4 are Staphylococcus sp. and 3 belongs to Archaebacteria. The strains were tested for their tolerance against six different types of heavy metals dominant in the ash samples viz. Pb, Hg, Ni, Co, Cu, Mn. Their maximum resistance existed upto 0.6mM/ml of the above mentioned different metals under lab standard conditions. Six isolates are found suitable for the multiple metal resistance ability viz SM 2, 3, 4, 5, 12 and 16. These are categorized as Bacillus cereus (SM 2,3, 4, 5), Bacillus subtilis (SM 12), Bacterium KKCSSM (SM 16) after performing 16S rDNA sequencing.
Keywords: MIC, 16S rDNA sequencing, heavy metal, ash dyke.
INTRODUCTION
Global pollution is increasing due to the variations in natural and anthropogenic activities, leading to contaminations in various aquatic and