Microbial Survey, Smear Preparation, and Simple Stain Essay

1271 WordsAug 13, 20136 Pages
MICROBIAL SURVEY, SMEAR PREPARATION, AND SIMPLE STAIN Instructional Objectives 1. Define Roccal = green, liquid disinfectant. Pathogen = an agent which causes disease. Wet Mount Slide = a microscope slide of a liquid specimen covered with a cover glass. Yeast = a single celled fungi. Budding = a true characteristic method of asexual reproduction among yeasts where budding of a new cell from a parent cell can be observed. Mold = multicellular masses of filamentous fungal growth. Hyphae = individual filaments of mold, generally comprised of more than one cell. Mycelium = the entire mass of the intermeshed hyphae. Colony = the sometimes circular body of fungal growth that is visible to the unaided eye. Can be comprised of…show more content…
To prepare a wet mount slide you begin with the substance at hand. The specimens studied in the laboratory using this type of slide were a hay infusion, a yeast suspension, and the mold specimen. For the hay infusion you begin with placing two drops of the suspension in the center of a clean microscope slide using a transfer pipet. The specimen must be immediately covered with a cover glass completing the wet mount slide. The yeast suspension is transferred from the tube to the slide using a flame sterilized inoculating loop. Immediately cover the specimen with a cover glass. The stained yeast suspension is prepared the same way except that the suspension is mixed with a drop of lactophenol cotton blue placed on the slide prior to transferring the yeast. The mold must be cut from the petri plate and placed on top of the drop of lactophenol cotton blue already placed on the microscope slide. After it is covered it may be studied under the microscope. 3. State the scientific name of the yeast studied in the laboratory. Saccharomyces cerevisiae is the scientific name of the yeast studied in the laboratory. 4. Name the medium upon which the mold was cultured. Sabouraud agar is the medium upon which the mold was cultured. 5. Name the stain routinely employed on fungal specimens. Lactophenol cotton blue is a stain routinely used on fungal specimens. 6. List two methods by which the mold specimen was examined. The mold

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