Microbiology Case Study Essay

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Introduction to Microbiology – Case Study 1
Kristen Alejos

You are working as an emergency room nurse in Topeka when a mother brings in her 8 year old son because of severe, bloody diarrhea. When questioned, the boy said that it hurts when he goes to the bathroom and his mother said he had vomited in the previous two days. When asked about their previous movements, the mother and son both mentioned a backyard barbeque that had taken place 6 days earlier. The boy complained that his mother made him eat a “pink” hamburger that was not thoroughly cooked. A physical examination of the boy revealed nothing out of the ordinary except dehydration from the diarrhea. Lab tests showed that he had 13,000 white blood cells/microliter of
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(3 pts) One important virulence factor produced by E. Coli is toxins. Toxins are proteins secreted by the bacteria that are poisonous to the host. In this case the toxins that are produced by E. Coli, damage the lining of the small intestine and that is what causes the bloody diarrhea. Sources: -Leboffie, Michael J. Microbiology. Burton E. Pierce. Morton. Englewood, Co. 2012

3. Based on what you know about the selective and differential capabilities of MacConkey agar, would it be possible to obtain similar results from a stool sample of a health patient? Why or why not? (3 pts) The MacConkey agar is capable of growing E. Coli because it inhibits the growth of Gram-positive bacteria and E. Coli is a gram-negative bacteria. If we took a stool sample from a healthy patient the selective media would be similar because E. Coli always grows on a MacConkey agar and E. Coli is always present in the human intestines. However the differential media would not be similar because of the amounts of E. Coli in the intestines and also whether it is infectious or not. Different kinds of E. Coli have the ability or inability to ferment lactose which changes the colors of the dyes after the incubation period. Sources: -Leboffie, Michael J. Microbiology. Burton E. Pierce. Morton. Englewood, Co. 2012

4. How is this organism spread? How can infection be prevented? (3 pts) According to the Mayo Clinic Staff, E.Coli is most easily

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