Essay on Microbiology

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You are given a sputum sample from a patient that may be infected with a bacterium from one of the following genera: Bacillus, Escherichia, or Mycoplasma. In a Microsoft Word document, describe the important anatomical differences among these three genera (i.e., those features that could be used to differentiate among them) and the staining protocols you would use to identify which genera is causing the patient’s infection. When discussing each staining protocol, it is important not only to mention the steps involved, but also how each step and each protocol would identify or eliminate each of these genera as a suspect. You may refer to other scientific resources, but they should be in addition to and not in place of the Module 2…show more content…
If there is question of the two types of bacterium being located in the specimen then the addition of the counterstain safranin. As the procedure continues alcohol is introduce to the sample to differentiate the Gram-negative and the Gram-positive bacterium with it causing the outer layer of the Gram-negative cells to dissolve allowing the counterstain to remain to mask to crystal violet color making the cells show as pink or red vs. purple. In particular cases of staining of Bacillus, some Gram-positive cells will give a Gram-negative response though they are dead, there can be increasing numbers of Gram –negative cells; this is called Gram-variable. (Tortora, 2014) The procedure relating to staining for observation of Mycoplasmas involve a detailed and specific protocol for success. As previously mentioned, Mycoplasmas have little to no cell wall; the staining process has to be relative to what can be stained for observation. In my research, I found that the use of a specific stain, the Hoechst #33258 dye, is used to illuminate by florescence the DNA of Mycoplasma bacteria so it can be differentiated from the remainder of the sample by the use of a UV filtrated, florescent microscope. (Sciences, 2002) The opportunity of each of the given genera that could cause this particular

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