Microbiology Labs

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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________

TITLE: Observing Bacteria and Blood

OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood.

PROCEDURES: Using the microscope, an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood:

After reviewing the section of the manual as instructed I cleaned the ocular lenses and prepared the slides. I made required adjustments to the microscope and ocular distances for view during experiment. I practiced using six prepared slides that were in the kit to ensure I was viewing
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I examined all three slides under the microscope. After observing the slides I flushed the specimens down the drain and disinfected the area with bleach solution. After 42-72 hours I assessed the growth patterns of the tube and noted that the tube that contained the swab became yellow stained and the tube that contained the agar and tablet dissolved a little and some of the content drifted to the surface of the tube and created a film.

1. There was no change from the fresh yogurt and the cultured yogurt. After 24 hours of sitting in a dark environment it became fermented and liquidy.

Mouth Swab - looks like coccobacillus; plaque: looks like single baccilus; Thick multi-layered – positive gram stain; colorless moving particles that resemble droplets clustered together
Yeast: looks like tetrad; moving particles, no stain; colorless moving particles
Plaque: thick multi-layered clusters – gram positive stain; solid stable particles, non moving Were you able to identify specific bacterial morphologies?
Yes I was able to identify some examples as I have previously stated.

What is the difference between direct and indirect staining? Did the smears appear different in each type of staining? Why or why not?
Direct Staining involves crystal violet and staining bacterial cells causing a negative reaction; Indirect staining involves staining but results are positive with pink solutions allowing bacteria to be viewed more accurately. These reactions are

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