Microscope Analysis : Microscope And Mitosis Prelab. Christie Jansen. Arapahoe Community College

In this experiment first the stages of an onion cell undergoing mitosis are going to be observed and every stage is going to be detected and drawn on paper. A brief description to what is going on should be attached to the pictures. This is important to understand the basics of cell division which is necessary growth,repair and asexual reproduction. Second the number of cells undergoing each phase is going to be counted to figure out in which phase the cell remains the most. If interphase is the stage in which the cell grows and prepares for cell division then the

Concept 6.1 Biologists use microscopes and the tools of biochemistry to study cells 1. The study of cells has been limited by their small size, and so they were not seen and described until 1665, when Robert Hooke first looked at dead cells from an oak tree. His contemporary, Anton van Leeuwenhoek, crafted lenses and with the improvements in optical aids, a new world was opened. Magnification and resolving power limit what can be seen. Explain the difference. Magnification is the ratio of an object’s image size to its real size. Resolution is a measure of the clarity of the image; it is the minimum distance two points can be separated and still be distinguished

Interphase: This particular stage is divided into three phases, G1 (first gap), S (synthesis), and G2 (second gap). During all three phases, the cell grows by producing proteins and other organelles within the cytoplasm. However, chromosomes are replicated only during the S phase. In all, a cell grows (G1), continues to grow as it duplicates its

Preparing specimen for electron microscope hard, light microscope still very useful as a window on living cells.

1) Apply the stain to your first unknown slide and examine it under the microscope.

The illuminating parts of a microscope enable us to see the detail of the subject placed under the microscope. The three main parts that enable us to do this are: the condenser which illuminates the object that is placed under the microscope, the objectives which forms the magnified image, and the eyepiece which enables us to see the magnified

Loosen the retaining screw and adjust the horizontal arm to the dimple that produce the 2nd largest radius between the spinning mass and the rotating shift. Allow the spinning mass to hang straight down, without being connected to the spring. Position the pointer directly below the tip of spinning mass and secure the pointer to the base. Use the scale located on the base to determine the radius, r, of the pointer from the rotating shaft. Record this value onto Data Sheet A. The experimental uncertainty in r is estimated to be the width of the spinning mass tip – approximately 0.2 cm.

It was then during the 1950’s that Zacharias Jansen and his father, Han Jansen, created the first high-powered compound light microscope. Images could be magnified up to 9x which was a novel feat at that time. Zacharias and his father created the device after experimenting with glasses and discovered that by placing multiple lens in a tube they could increase magnification. Unfortunately, the microscope lacked the high resolution we have come to expect in modern-day light microscopes. The images produced by the Hans’ primitive microscope was extremely blurry and had limited

[A labelled diagram of the end of the Prophase stage of a cell here would be

Interphase is often called the resting stage of mitosis. It is a stage in the life cycle of a cell where, putting it simply, DNA is replicated and each

The height and width of this optotype is designed to be five times the thickness of the stroke width and gap width, therefore the size of each critical detail is 1/5th of the overall height (Kalloniatis & Luu, 2005). Four stimulus sizes were used in the experiment as visual acuity was to be tested at four eccentricities (0˚, 20˚, 40˚ and 60˚). These four stimulus sizes were calculated using the approximate minimum angle of resolution (MAR) of each eccentricity obtained from Millodot et al.’s (1975) normal data. This value was then used to find the gap size and hence the stimulus size using a distance of 600cm. The reason for using the distance value of 600cm was because it is close to the threshold and therefore would be the starting point of stimulus presentation for each of the eccentricities. The formula used was:

Mitosis is an important process that it is responsible for mostly everything that goes on in your body. It allows one cell with 46 chromosomes to split into two that are genetically identical. The nucleus is in the center of the cell in which all DNA is stored. So in reality you don't need to duplicate the entire cell, only the DNA. In addition, mitosis takes place in stages called prophase, metaphase, anaphase, and telophase. Before mitosis all cells are in interphase where they grow and work to become useful. During interphase, there is loose DNA inside the cell that is called chromatin. Then the centrosome replicates next to the nucleus. Afterword, the DNA begins to replicate itself to have two copies of every DNA strand. In the next phase called prophase, the chromosomes begin to condense and the mitotic spindle forms. Next, comes the longest phase of mitosis, metaphase, in which the nuclear envelope breaks apart, and the cells use motor proteins to allow the

Procedure: First, set up the microscope. Clean the ocular lenses and objectives with lens paper. Then pace the prepared e slide on the stage and make adjustments. Turn the rotating nosepiece until the 10x objective is above the ring of light coming through the slide. Move the slide using the X and Y stage knobs until the specimen is within the view. Adjust the focus by looking into the eyepiece and focusing the specimen with the coarse then fine focus knobs. Adjust diaphragm until there is sufficient light

The cell cycle is separated into multiple stages such as G0, G1, S, G2, and M phase. During the G0 phase is the stage in which there is no sign of the cell preparing to separate but, rather a stagnant cell. The G1 phase involves the expansion of the cell and gathering all the nutrients and energy to prepare for the upcoming processes of the cell cycle. In the S phase,

Most microscopes, including those in schools and laboratories today, are optical microscopes. They use glass lenses to enlarge, or magnify, an image. An optical microscope cannot produce an image of an object smaller than the length of the light wave in use. To see anything smaller than 2,000 angstroms (about 1/250,000 of an inch) a wave of shorter length would