Microscopy allows one to view samples and objects that cannot be seen with the human eye. With all

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Microscopy allows one to view samples and objects that cannot be seen with the human eye. With all the modern techniques used in the biology field today, microscopy is one of the most important one that allows us to see a world beyond what our eyes limits us too. The majority of both organisms that are helpful and harmful are too small to be seen with the human eye, and without the microscope we would have not understood the mechanism behind the major diseases we know today. Microscopy has helped scientists produce antibiotics and antiviral drugs to treat the diseases that once were in a world beyond our vision. There are three types of microscopy mainly used: optical (light), electron, and scanning probe microscopy. Optical…show more content…
Fluorescence microscopy uses ultraviolet light as its light source that allows the resolution of the object to increase. Fluorescence microscopy contains a barrier filter that reduces any undesired stray of light other than the emission wavelength that might reach the observers eye. Light emitted by the specimen’s fluorophore for viewing is focused and magnified by the objective lens. Electron microscopy uses an electron beam with a far smaller wavelength than Optical microscopy that results in better resolution one can achieve. This is due to the fact that as wavelength increases, resolution decreases. Electron microscopy allows for a resolution on the order of 1000 times greater than can be achieved with light microscopy. There are two types of Electron microscopy: Transmission and Scanning electron microscopy. Transmission electron microscopy (TEM) is similar to light microscopy by sending an electron beam through a very slice thin of specimen. TEM uses an electromagnet, a condenser lens, to focus a stream of electrons onto a specimen that produces images of internal structures in 2-D slices of a specimen. Scanning electron microscopy (SEM) produces 3-D image of the external structure of a specimen by observing the surface of unsectioned samples. This is possible through the specimen being lightly coated with a heavy metal that is constantly scanned by a stream of electrons to produce the image

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