Mitochondrial Dna And Its Effect On Human Behavior Essay

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Introduction
Plasmid was a term introduced by Lederburg in 1952 as a descriptive for genetic material that is located outside of the nucleus of a cell at points or the entirety of its replication cycle and origionates from and returns to chromosomal DNA (Lederberg, 1952). In 1968 this descriptive was adapted to being elements of the genetic material that are generally located outside of the chromosome and can carry out self-replication. recognition that it also didn’t cause cell damage which highlighted that it was different from a virus (Lederberg, 1998). The features of plasmids such as size, being extrachromosomal along with the ability of self-replication provide a basis for manipulation of genetic material alongside the ability to analyse the roles of different variations. In this report it is shown how certain techniques are carried out along with the reasoning behind the methodology to isolate and manipulate plasmids within E.coli. The initial step in the protocol was to isolate the plasmid DNA, plasmid DNA is useful when conducting experiments involving gene manipulation this is due to the determination of plasmid and chromosomal DNA. Plasmid DNA generally do not contain genes that are needed for the cells regulation (housekeeping), a genetic element that contains the genes for the cells regulation would be classified as chromosomal DNA. Due to plasmids not carrying DNA responsible for the cell to survive normal conditions they provide a gateway toward the production
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