Molecular And Extinction Of Fear Memories Of The Brain Associated With Contextual Fear Essay

882 Words4 Pages
C. APPROACH: Based on our preliminary data on lncRNA changes in specific regions of the brain associated with contextual fear, we hypothesize that specific lncRNAs mediate consolidation and extinction of fear memories. To test this hypothesis, the Puthanveettil and Stackman laboratories will bring together their strong expertise in genomics, bioinformatics, noncoding RNAs, genetics, behavioral models, non-coding RNAs, bioinformatics and functional, in vivo manipulations of novel molecular players. We will employ a research strategy that starts with an unbiased, genome-wide analysis of lncRNAs and funnel down to key players, at which point mechanisms of action will be identified. First, unbiased expression analysis of lncRNAs will be carried out with contextual fear conditioning following extinction of contextual fear memory. Second, we will then carry out in vivo manipulation of specific lncRNAs in specific regions of the brain to determine necessity of the expression of specific lncRNAs in consolidation and extinction of fear memory. We anticipate that our studies will establish the functional importance of candidate lncRNAs in consolidation and extinction and provide novel insights into the novel molecular regulators and mechanisms underlying development of PTSDs. All of the genomics, bioinformatics and rodent behavior analyses will be carried out locally, at Scripps Florida and FAU, with confocal imaging experiments being performed across the street at the Max Planck

    More about Molecular And Extinction Of Fear Memories Of The Brain Associated With Contextual Fear Essay

      Get Access