Molecular Biology is the Transcription from DNA to RNA

1105 Words Feb 26th, 2018 4 Pages
In these past few experiments, the students looked at mRNA, treated them with PMA and DMSO. The students then performed reverse transcriptase on their mRNAs in order to convert them into cDNA, which is more stable. After that, the students then performed PCR on their cDNA to zero in on the gene of choice. Those genes were then run through gel electrophoresis. The reasons behind these particular processes were to try to isolate the genes MMP-9 and beta-actin in both the DMSO and PMA groups. PCR (polymerase chain reaction) is used to zero in on a certain gene of choice. First, the DNA is heated to 55C, and then heated to 95C, which splits the double-stranded DNA into single-stranded DNA. The mixture is then brought back down to 55C in which specific primers anneal to the strands of DNA. Once that process is completed, the mixture is then heated to 72C, which causes TAC polymerase to grab onto random oligonucleotides. Finally, the mixture is brought back up to 95C to allow the DNA to split again. This process repeats a total of 32 times resulting in millions of copies of the gene in question. The primers added to the mixture only attach to specific sequences in order to insure that the gene the experimenter wants is the only one that copies. The process of PCR allows for the proliferation of the gene in question to reach a high…