Molecular Genetic Experiment : Biology Lab

1793 WordsMar 22, 20178 Pages
Molecular Genetic Experiment: Exploring the Expression of an Auxin Induced Gene pCNT103, a Constitutive Gene GapC and a Cytokinin Induced Cig1 Gene in the DNA and RNA Extracts of Differentiated Shoot, Root, and Callus Tissue of Nicotiana tabacum Demaris Gonzalez Genetics Lab Professor Kamil Starczak March 23rd , 2017 Abstract: The experiments purpose was to understand and observe the gene expressions in the genes pCNT103, cig1 and GapC in the shoot, root and callus tissues of the tobacco plant, Nicotiana tabacum. Using various genetic laboratory research techniques completed the experiment. The experiment consisted of four parts. The sterile tissue culture technique was used to differentiate the tobacco callus tissue in…show more content…
Molecular genetics is the study of the chemical nature of the gene itself while genetics, as a whole, is the study of heredity and inherited characteristics. Molecular genetics is more so a branch of the genetics study and expresses how genes and genetic information is encoded, replicated, and expressed. There is a depth induction in the study of molecular genetics to how the processes of replication, transcription, translation, and gene regulation happen and are completed. The study exemplifies how genes are units of heredity that passed down from generation to generation and they encode certain characteristics (Pierce, 2014). The tobacco plant like many plants contain a cell callus. A cell callus contains somatic undifferentiated cells and can be used to differentiate into specialized tissues of the tobacco plant, or any plant used, by being induced with the addition of different types of hormones, such as cytokinin and auxin. Cytokinin and auxin are mostly used in plant tissue culture simultaneously to provoke the formation of a plantlet or callus. There is a common use of Kinetin in plant tissue culture since when added it will promote cell division to initiate shoot tissues from calluses of the plant. Kinetin is a type of cytokinin hormone. In regards to auxin related hormones, Indole-3-acetic acid (IAA) is also commonly used since it promotes the initiation of roots for the root tissue of the plant. In this
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