Molecular Genetics 16s Lab Report Essay

2106 WordsDec 1, 20169 Pages
Molecular Genetics 16s Lab Report Abstract A highly conserved gene will be used to identify a prokaryotic species isolated from the body. Fundamental lab techniques will be also explored and utilized, such as amplifying using PCR, cloning, and transforming the gene into a host cell. DNA electrophoresis and specific substrate plating will serve as analysis check points. The final product will be sequenced and compared to similar species to observe phylogenetic relationships. Intro Bacteria have an intrinsic role to humans thriving commensally in and outside the body, despite their evolutionary and structural differences from us. Their prokaryotic classification gives insight to methods used in identification and observation. Researchers can pinpoint certain genes they know are vital across their phylogeny. An example of this would include autosomal genes, like those that encode for structural proteins used in translation. Particularly, the 16s rRNA gene is highly conserved across prokaryotic species, but unlike many conserved genes, it also includes small variable regions specific to each species. This makes the 16s gene useful as an identification signature for prokaryotes, and it is the most common genetic marker used today (Janda). This is not only due to its presence in nearly all prokaryotes, but because it has been genetically unchanged over time, for the most part. However a flaw to using the 16s rRNA gene is that it cannot be a definite identification
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