Morphologic Changes of the Cells Infected with Rubella Virus
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Morphologic changes of the cells infected with rubella virus cannot be observed easily. Estimation of the size of the cultured cells can be a valuable parameter in this condition. Therefore, detection of morphological changes of the cells or nuclei induced by the virus before observing any later marked changes can be beneficial. Briefly, the study was conducted to find answers to the following questions. How much time after infecting with rubella virus the volume and surface area of the Vero cells and their nuclei get started to change? How it possible to apply stereological methods to estimate the volume and surface area of the cultured cells using the invariator, nucleator, and surfactor techniques?
Methods: The cultured Vero cells were infected with rubella virus. The cells of the control and experimental groups were harvested at 2, 4, 8, 24, and 48 hours following the incubation period. The cells were processed and embedded in paraffin. Then, isotropic uniform random sections of the cells were obtained using ‘‘isector’’ method. Invariator, nucleator, and surfactor were applied to estimate the size of the Vero cells and their nuclei.
Results: The cell volume was decreased by 15-24%, 48 hours after the infection in comparison to the non-infected cells. Besides, the cell surface area was decreased by 13% 48 hours after the infection. However, no changes were detected in the nuclei. The values of the standard deviation and coefficient of variation of the cells estimated by