Motifss : Understanding The Specific Functions Of DNA Motifs

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What are regulatory-motifs? They are incredibly powerful strings of amino acids called promoters which help regulate whether an important gene function is turned on or off. There are thousands of motifs that have been discovered; however, understanding the specific function of a particular motif is difficult. When analyzing a DNA sequence, it is challenging to see where in the segment the motifs occur. Thanks to bioinformatics, algorithms have been developed to assist in finding motifs. A sequence run through a program could look like this:

Figure 1: example of motif in DNA sequence. {The locations of the motif are highlighted in green.) With an algorithm we can locate the motif much faster to analyze it. Once the motif is found,
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Cystic fibrosis is a life-limiting disorder which causes sticky secretions in the lungs and in the ducts of the pancreas. Children born with this disorder face a lifetime of struggling to breathe, and treatments consisting of percussion on the back to loosen the mucous. In addition, these children develop diabetes as they get older. There is very little quality of life for these youngsters. The discovery of F508del in the 90s has helped to change that outlook. This is the transmembrane conductance regulator gene (CFTR) which was found to be the most common cause of cystic fibrosis. It functions as a chloride channel regulated by cyclic AMP dependent phosphorylation. Almost 2,000 variants of this gene have been reported, 40% are predicted to cause substitution of a single amino acid, and 36% are expected to alter RNA processing. This has led to research into the possibility of genetic modifiers, which if successful could either improve the severity of, or eliminate this disorder. The CFTR gene in mice has been successfully manipulated to derive lines that do not expresses CFTR, and lines that express CFTR bearing variants equivalent to those expressed in humans. The absence of lung disease similar to that seen in humans with cystic fibrosis has been ascribed to the presence of alternative pathways for chloride transport in mouse epithelial cells.
Algorithms are

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