Mycelial Pathogen

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Step 1: Written observation of the symptoms of the fruit and signs of the pathogen.
Symptoms and signs
The fruit that is examined in this study is a strawberry. The infected strawberry in displayed in figure 1, appears to be fully covered in mycelial growth. Mycelial consists of white filaments also known as hyphae (mould), which seems to be growing on the strawberry. Within the branched hyphae there are multiple black spores that surround the fruit. Although the fruit appears to be shriveled, there appears to be an access amount of liquid which remains on the parafilm and the inner components of the fruit are moist and soft. The multiple stages of mycelial that are growing on the fruit in figure 1, demonstrates signs of the pathogen. The
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Sterilize the spatula by holding it over the flame, after cooling proceed to cut 5 mm square block of agar from the unioculated petri dish. Following this step the square of agar was transferred to the centre of the microscope slide. Next, using both the sterile forceps and a scapula a collection of a portion of mycelia mass from the culture and inoculate on both the top and the bottom of the agar cube. After re-flaming and cooling of the spatula and forceps, a sterile cover slip was carefully placed on the upper inoculated surface over the infected agar. The final process includes incubating the closed dish at room temperature until growth was visible (approx. 2-4 days). If there is no visible growth, allow for mould to grow for another 24-48 hours. The closed dish of culture was placed in the fridge until next the session. The new healthy fruit appears to be a ripe, rich red color, with a solid/firm texture and no signs of…show more content…
The slide appears to attain dark hyphae fruiting bodies at the tips. The appearance of spores, sporangiospore and sporangium are visible. Rhizopus is identified in the microscopic analysis of the fruit. The rhizopus is seen as the branching hypha ends in figure 4. The microscope slide demonstrates density of hyphae around the mid region.
Do the Pure Culture Symptoms Observations Match? At the beginning of the experiment, the samples had visible hyphae and black fruiting bodies, which appear to be visible in the re-infected fruit samples. Both the samples have distinct zones of mycelial growth and colonization. In the fruiting and productive zones both samples appear to have increased hyphae density. When samples are analyzed in the microscope they both have evident clusters of sporangia and conidia. When comparing the infect fruit samples it is clear that both the original and re-infected samples are quite similar results including signs and symptoms.
Step 5: Re-isolation of the pathogen in a pure culture and compare to original
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