Negative Separation Of 50 Ml Plastic Tubes

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To prepare experiment 1, labels were put on four separate 50 mL plastic tubes. They were each labeled, “Negative control A”, “Positive control B”, “Positive control”, and “Experimental”. Fermentation tubes were also labeled in the same way as the vials. Next, glucose in the amount of either 2% or 6% and water was extracted from beakers using a pipette pump. The water and glucose were then mixed in the plastic tubes before adding the yeast from a separate beaker, also extracted by a pipette pump. It was important to make sure that the yeast is added last, as the reaction will happen as soon as yeast and sugar come into contact. After the solutions were mixed together, they were put into their previously labeled fermentation tubes. After the…show more content…
The plastic tubes were again filled with water and sucrose or glucose depending on the tube label using pipette pumps, and then yeast was added to the mixture (see “Table 3”). In tube 1 “Negative control A”, 4mL of 6% sucrose was added to 8mL of water, while 0mL yeast was added. In tube 2 “Positive control B”, 4mL of yeast was added to 8mL of water, while 0mL sucrose was added. In tube 3 “Positive control”, 4mL of 6% glucose and 8mL of water were mixed together before 4mL of yeast was added to the mixture. Lastly, in tube 4 labeled “experimental”, 4mL of 6% sucrose was added to 8mL of water before 4mL of yeast was added. After all of the plastic tubes were mixed, they were poured into the fermentation tubes. Again, all of the air had to be removed by holding a thumb over the opening and slowly tilting the tubes sideways. The tubes were then left to sit for 30 minutes before the air bubbles were measured using a ruler.

Table 3: Experimental Design-Addition Of Sucrose: Tube 1 and 2 are the negative control A and positive control B. Tube 4 was the experimental as sucrose was added instead of glucose to test the amount of fermentation. Tube 3 was the positive control to compare the experimental (tube
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