Ninhydrin Research Paper

7. Tape the strip to a pencil and rest the pencil on top of the jar so that the strip hangs into the jar. The goal is to have the end of the chromatography strip just touching the surface of the solvent solution, with the colored dots above the surface of the liquid. Make sure that the colored spots do not come in direct contact with the liquid in the bottom of the glass.

It is a chemical reaction where the colors of both solution and iron strip changed. It is

They first had to identify whether the body was indeed Tiernan’s or not and two days after the body was found, fingerprinting methods indeed determined that the body was Leanne Tiernan. Despite her body being found 9 months later, her body was still intact enough to gather fingerprints. Fingerprinting proves to be a critical method in forensic cases as no two individuals have been recorded having the same fingerprint, making it an ideal way to identify suspects or victims. Fingerprints are also individual characteristics making it nearly 100% chance that it could be traced back to a source. Getting something Tiernan may have touched can then be compared to a fingerprint(s) taken from her body. Fingerprints have ridge characteristics known as minutiae and can also be classified on their general pattern known as loops, arches, and whorls. Fingerprints also do not change unless damage is done to the dermal papillae, which makes fingerprints a powerful use of evidence. Extracting a fingerprint from Tiernan would require pressing a finger into an ink pad and then transferring the impression onto a card that would be examined by a professional. However, getting a sample to compare her fingerprint would require investigators to find a latent fingerprint (prints that are left on a surface which are invisible to the naked eye) of Tiernan. When we touch something, we leave impressions on surfaces due to the sweat and oil and as long as the

Any moist or wet biological evidence (blood, body fluids, plants, etc.) like the lottery ticket on the counter with the blood smeared print on it should be collected wearing gloves it should then be placed in a clean unused paper container such an envelope, and or small bag and transported back to an evidence receiving area, it should then be taken out of the bag and allowed to air dry thoroughly. The Ninhydrin process should be applied to this piece of evidence to obtain noticeable prints.

In quadrants 1 and 2 how the amount and constituents of the fingerprint residue on the substrate affects the fingerprint image, is determined. In quadrant 1, excess sebum and moisture is first removed from the finger tips with the help of a clean cloth. In quadrant 2, fingertip is first wiped around the nose or forehead to create excess sebum. Quadrant 3 and 4 were used to compare the details between untreated and dusted fingerprint residues.

If you put too much of a sample on your chromatography paper, you could possibly have that color bleed into the color next to it, which would mess up your results. If you put too little of a sample on the paper, your color

Then we carefully submerged the nitrocellulose sheet on the transfer buffer for about 5 minutes, and set up the transfer apparatus in the method shown in the lab outline week 2 step 3-6. Afterwards we took apart the transfer apparatus and detached the nitrocellulose sheet. Then we put the nitrocellulose sheet for 5 minutes into the blocking buffer, and we left nitrocellulose sheet in a container till week 3. The second part of week 2 was staining half our PAGE gel from week 1. Which consisted of pouring deionized water and use the shaker machine for 5 minute intervals, and repeating the the process three times. Then the Fresh Coomassie stain was added to the gel and was rinsed (UFV BIO 202 Lab,

The wet blood should be transferred onto clean cotton cloth. Bloodstained cotton cloth must be allowed to air dry before packaging in a paper container. Each object and container must be properly labeled. Dried bloodstains on weapons, for example, should be collected separately by collecting the entire item. Each item should be placed in its own paper container, and these should be sealed and labeled properly. The bloodstain pattern should be documented and sketched to the extent necessary. The stain can be tape lifted or scraped off the object onto a clean piece of paper. The tape lifter or the paper with blood can then be placed into a druggist fold, and placed in an envelope which is sealed. Each item must be labeled

Method used: I used my latent fingerprint dusting powder in order to bring the print to a visible state to the naked eye and then I used my tape I had to gain a copy of the print off the handle. I then took the evidence to my forensic vehicle in order to keep it secure for further examination.

1. The 1% saltwater solution was easy to create, however there was a greater amount of this solution than the alcohol. The saltwater solution worked better and had more effect on each of the colors on the strip of paper than the alcohol solution because the colors began

Thin Layer Chromatography (TLC) works in relation to the polarity of chemicals. A plate is first covered with aluminum foil or silica etc. and has solutions of varying polarities placed upon the aluminum foil or silica. When placed in a in a puddle of solvent that moves up the plate, the different inks i.e. the solution will move up the place based on their Rf values. Adherence increases with increasing polarity, so the less polar compounds will be carried farther by the solvent. Eventually the dyes will separate into their compontnets, which can be visibly seen. This is then used to determine who the ink of the unsigned note belonged to along with the pen that it belonged

For this lab I had to prick my finger, then with the bleach solution I had to dab it and then carefully drop the blood on the slide. Once I was finished I had to take the second slide and smear the blood. I had to let the slide dry. Then I had to prick my finger again and make 3 more slides. Then I had to mix the chemical with a toothpick and then look under with the microscope.

Add 4 drops of phenolphthalein in to the prepared acetic acid, and check whether the color changes to dark pink.

The next step was to place the strip of chromatography paper on a paper towel. Then dip a capillary tube into the plant pigment extract (spinach pigment extract) provided by the teacher. The tube will fill on its own. We applied the extract to the pencil line on the paper, blew the strip dry, and repeated it three to four times until the line on the paper is a dark

Fingerprint patterns are classified into three main categories: loops, whorls, and arches. Loops make up sixty percent of the prints that are left behind, and their patterns trace back onto themselves, pointing either towards the thumb or the pinky. Whorls make up thirty-five percent of prints left behind, and they are circular in nature. These have many classifications and patterns: they can be concentric circles, or plain whorls; they can be loops with whorls at